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Assay Detail

CHEMBL5041569

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-[Nle75,Tyr77][Pyr1]-Ape13 from YFP-tagged human APJ expressed in HEK293 cell membranes assessed as inhibition constant incubated for 1 hr by scintillation counting method
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apelin receptor (CHEMBL1628481)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Size-Reduced Macrocyclic Analogues of [Pyr<sup>1</sup>]-apelin-13 Showing Negative Gα<sub>12</sub> Bias Still Produce Prolonged Cardiac Effects.
Tran K, Sainsily X, Côté J, Coquerel D, Couvineau P, Saibi S, Haroune L, Besserer-Offroy É, Flynn-Robitaille J, Resua Rojas M, Murza A, Longpré JM, Auger-Messier M, Lesur O, Bouvier M, Marsault É, Boudreault PL, Sarret P.
J Med Chem (2022) 65 : 531 -551
PubMed 34982553 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 33 7.72 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5094438 1 9.70
CHEMBL5091501 1 9.22
CHEMBL5087847 1 9.22
CHEMBL5078149 1 9.05
CHEMBL5092962 1 8.77
CHEMBL5091081 1 8.77
CHEMBL5072626 1 8.60
CHEMBL4083079 1 8.52
CHEMBL5080649 1 8.37
CHEMBL5079665 1 8.34
CHEMBL5074959 1 8.28
CHEMBL5085866 1 8.24
CHEMBL5092604 1 8.07
CHEMBL5082518 1 7.85
CHEMBL5089609 1 7.80
CHEMBL5079005 1 7.55
CHEMBL5093180 1 7.48
CHEMBL5093858 1 7.41
CHEMBL5076304 1 7.21
CHEMBL5090040 1 7.07
CHEMBL5074666 1 7.07
CHEMBL5088099 1 6.96
CHEMBL5082011 1 6.84
CHEMBL5091385 1 6.77
CHEMBL5070767 1 6.70
CHEMBL5091291 1 6.46
CHEMBL5079790 1 6.40
CHEMBL5081025 1 5.69
CHEMBL5079641 1 5.57
CHEMBL5075292 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5094438 Ki = 0.2 nM 9.70
CHEMBL5091501 Ki = 0.6 nM 9.22
CHEMBL5087847 Ki = 0.6 nM 9.22
CHEMBL5078149 Ki = 0.9 nM 9.05
CHEMBL5092962 Ki = 1.7 nM 8.77
CHEMBL5091081 Ki = 1.7 nM 8.77
CHEMBL5072626 Ki = 2.5 nM 8.60
CHEMBL4083079 Ki = 3.0 nM 8.52
CHEMBL5080649 Ki = 4.3 nM 8.37
CHEMBL5079665 Ki = 4.6 nM 8.34
CHEMBL5074959 Ki = 5.3 nM 8.28
CHEMBL5085866 Ki = 5.7 nM 8.24
CHEMBL5092604 Ki = 8.6 nM 8.07
CHEMBL5082518 Ki = 14.0 nM 7.85
CHEMBL5089609 Ki = 16.0 nM 7.80
CHEMBL5079005 Ki = 28.0 nM 7.55
CHEMBL5093180 Ki = 33.0 nM 7.48
CHEMBL5093858 Ki = 39.0 nM 7.41
CHEMBL5076304 Ki = 61.0 nM 7.21
CHEMBL5090040 Ki = 86.0 nM 7.07
CHEMBL5074666 Ki = 86.0 nM 7.07
CHEMBL5088099 Ki = 109.0 nM 6.96
CHEMBL5082011 Ki = 143.0 nM 6.84
CHEMBL5091385 Ki = 170.0 nM 6.77
CHEMBL5070767 Ki = 201.0 nM 6.70
CHEMBL5091291 Ki = 343.0 nM 6.46
CHEMBL5079790 Ki = 396.0 nM 6.40
CHEMBL5081025 Ki = 2044.0 nM 5.69
CHEMBL5079641 Ki = 2670.0 nM 5.57
CHEMBL5075292 Ki > 10000.0 nM -
CHEMBL5077256 Ki > 10000.0 nM -
CHEMBL5094794 Ki > 10000.0 nM -
CHEMBL5075513 Ki > 10000.0 nM -