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Assay Detail

CHEMBL5042273

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full length N-terminal GST-fused MPS1 (1 to 857 residues) using histone H3 as substrate by TR-FRET assay
6
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity protein kinase TTK (CHEMBL3983)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of the First Covalent Monopolar Spindle Kinase 1 (MPS1/TTK) Inhibitor.
M Serafim RA, da Silva Santiago A, Schwalm MP, Hu Z, Dos Reis CV, Takarada JE, Mezzomo P, Massirer KB, Kudolo M, Gerstenecker S, Chaikuad A, Zender L, Knapp S, Laufer S, Couñago RM, Gehringer M.
J Med Chem (2022) 65 : 3173 -3192
PubMed 35167750 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.98 8.04
Ki 1 9.08 9.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5094006 2 9.08
CHEMBL5077230 1 8.04
CHEMBL2140523 1 8.01
CHEMBL4449557 1 -
CHEMBL4456085 BAY-1217389 1.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5094006 Ki = 0.83 nM 9.08
CHEMBL5077230 IC50 = 9.06 nM 8.04
CHEMBL2140523 IC50 = 9.79 nM 8.01
CHEMBL5094006 IC50 = 13.0 nM 7.89
CHEMBL4449557 IC50 < 1.0 nM -
CHEMBL4456085 BAY-1217389 IC50 < 1.0 nM -