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Assay Detail

CHEMBL5042809

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type full length DYRK1A (unknown origin) in human U2OS cells assessed as reduction in pSER520 autophosphorylation incubated for 5 hrs by western blot analysis
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Structure-Guided Discovery of Potent and Selective DYRK1A Inhibitors.
Weber C, Sipos M, Paczal A, Balint B, Kun V, Foloppe N, Dokurno P, Massey AJ, Walmsley DL, Hubbard RE, Murray J, Benwell K, Edmonds T, Demarles D, Bruno A, Burbridge M, Cruzalegui F, Kotschy A.
J Med Chem (2021) 64 : 6745 -6764
PubMed 33975430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.88 7.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5093661 1 7.58
CHEMBL5070553 1 7.55
CHEMBL5089171 1 7.50
CHEMBL5077927 1 7.47
CHEMBL5088511 1 7.46
CHEMBL5084166 1 7.36
CHEMBL5080497 1 7.33
CHEMBL5071425 1 7.14
CHEMBL5084504 1 7.10
CHEMBL4460561 1 7.05
CHEMBL5077422 1 7.05
CHEMBL5082456 1 7.00
CHEMBL4575900 1 6.96
CHEMBL5072926 1 6.96
CHEMBL5091644 1 6.89
CHEMBL5092833 1 6.88
CHEMBL5092736 1 6.82
CHEMBL5073976 1 6.65
CHEMBL5094409 1 6.64
CHEMBL5088553 1 6.60
CHEMBL5089294 1 6.55
CHEMBL5092362 1 6.30
CHEMBL5073869 1 5.78
CHEMBL5094822 1 5.75
CHEMBL5073581 1 5.52
CHEMBL5079873 1 -
CHEMBL5090552 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5093661 IC50 = 26.0 nM 7.58
CHEMBL5070553 IC50 = 28.0 nM 7.55
CHEMBL5089171 IC50 = 32.0 nM 7.50
CHEMBL5077927 IC50 = 34.0 nM 7.47
CHEMBL5088511 IC50 = 35.0 nM 7.46
CHEMBL5084166 IC50 = 44.0 nM 7.36
CHEMBL5080497 IC50 = 47.0 nM 7.33
CHEMBL5071425 IC50 = 73.0 nM 7.14
CHEMBL5084504 IC50 = 80.0 nM 7.10
CHEMBL4460561 IC50 = 90.0 nM 7.05
CHEMBL5077422 IC50 = 89.0 nM 7.05
CHEMBL5082456 IC50 = 100.0 nM 7.00
CHEMBL4575900 IC50 = 111.0 nM 6.96
CHEMBL5072926 IC50 = 110.0 nM 6.96
CHEMBL5091644 IC50 = 128.0 nM 6.89
CHEMBL5092833 IC50 = 131.0 nM 6.88
CHEMBL5092736 IC50 = 153.0 nM 6.82
CHEMBL5073976 IC50 = 226.0 nM 6.65
CHEMBL5094409 IC50 = 228.0 nM 6.64
CHEMBL5088553 IC50 = 250.0 nM 6.60
CHEMBL5089294 IC50 = 280.0 nM 6.55
CHEMBL5092362 IC50 = 500.0 nM 6.30
CHEMBL5073869 IC50 = 1650.0 nM 5.78
CHEMBL5094822 IC50 = 1800.0 nM 5.75
CHEMBL5073581 IC50 = 3000.0 nM 5.52
CHEMBL5079873 IC50 > 300.0 nM -
CHEMBL5090552 IC50 - -