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Assay Detail

CHEMBL5042988

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of human V1A receptor expressed in human 1321N1 cells assessed as calcium mobilization by fluorescent plate reader
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type 1321N1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vasopressin V1a receptor (CHEMBL1889)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Characterization of New V<sub>1A</sub> Antagonist Compounds: The Separation of Four Atropisomeric Stereoisomers.
Szeleczky Z, Szakács Z, Bozó É, Baska F, Vukics K, Lévai S, Temesvári K, Vass E, Béni Z, Krámos B, Magdó I, Szántay C, Kóti J, Domány-Kovács K, Greiner I, Bata I.
J Med Chem (2021) 64 : 10445 -10468
PubMed 34255509 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 8.18 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4799793 1 9.00
CHEMBL5070261 1 8.96
CHEMBL5074709 1 8.92
CHEMBL5070075 1 8.74
CHEMBL5081323 1 8.72
CHEMBL3416885 RO-5028442 1.0 1 8.70
CHEMBL4297183 BALOVAPTAN 3.0 1 8.52
CHEMBL1837037 1 7.51
CHEMBL5085869 1 6.41
CHEMBL344159 TOLVAPTAN 4.0 1 6.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4799793 IC50 = 1.0 nM 9.00
CHEMBL5070261 IC50 = 1.1 nM 8.96
CHEMBL5074709 IC50 = 1.2 nM 8.92
CHEMBL5070075 IC50 = 1.8 nM 8.74
CHEMBL5081323 IC50 = 1.9 nM 8.72
CHEMBL3416885 RO-5028442 IC50 = 2.0 nM 8.70
CHEMBL4297183 BALOVAPTAN IC50 = 3.0 nM 8.52
CHEMBL1837037 IC50 = 31.0 nM 7.51
CHEMBL5085869 IC50 = 390.0 nM 6.41
CHEMBL344159 TOLVAPTAN IC50 = 470.0 nM 6.33