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Assay Detail

CHEMBL5043705

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Allosteric antagonist activity against mouse CB1 receptor expressed in HEK293 cells membrane measured after 60 mins by [35S]GTPgammaS binding assay
19
Total Activities
19
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cannabinoid receptor 1 (CHEMBL3037)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Development of 3-(4-Chlorophenyl)-1-(phenethyl)urea Analogues as Allosteric Modulators of the Cannabinoid Type-1 Receptor: RTICBM-189 is Brain Penetrant and Attenuates Reinstatement of Cocaine-Seeking Behavior.
Nguyen T, Gamage TF, Finlay DB, Decker AM, Langston TL, Barrus D, Glass M, Li JX, Kenakin TP, Zhang Y.
J Med Chem (2022) 65 : 257 -270
PubMed 34929081 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
pIC50 11 - -
IC50 8 5.88 6.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5080045 1 6.25
CHEMBL5092369 1 6.12
CHEMBL5087480 1 6.11
CHEMBL5077926 1 6.05
CHEMBL5080578 1 5.82
CHEMBL1289260 1 5.43
CHEMBL5079805 1 5.38
CHEMBL5087934 1 -
CHEMBL5082643 1 -
CHEMBL2299364 1 -
CHEMBL5084600 1 -
CHEMBL5088172 1 -
CHEMBL5081586 1 -
CHEMBL5093337 1 -
CHEMBL5086597 1 -
CHEMBL5087483 1 -
CHEMBL5089573 1 -
CHEMBL1360165 1 -
CHEMBL5077368 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5080045 IC50 = 562.34 nM 6.25
CHEMBL5092369 IC50 = 758.58 nM 6.12
CHEMBL5087480 IC50 = 776.25 nM 6.11
CHEMBL5077926 IC50 = 891.25 nM 6.05
CHEMBL5080578 IC50 = 1513.56 nM 5.82
CHEMBL1289260 IC50 = 3715.35 nM 5.43
CHEMBL5079805 IC50 = 4168.69 nM 5.38
CHEMBL5087934 IC50 > 10000.0 nM -
CHEMBL5082643 pIC50 - -
CHEMBL2299364 pIC50 - -
CHEMBL5084600 pIC50 - -
CHEMBL5088172 pIC50 - -
CHEMBL5081586 pIC50 - -
CHEMBL5093337 pIC50 - -
CHEMBL5086597 pIC50 - -
CHEMBL5087483 pIC50 - -
CHEMBL5089573 pIC50 - -
CHEMBL1360165 pIC50 - -
CHEMBL5077368 pIC50 - -