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Assay Detail

CHEMBL5044913

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]SCH-58261 from A2A adenosine receptor (unknown origin) expressed in HEK cell membrane incubated for 60 mins at room temperature by NXT microplate scintillation counter analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of 2-Amino-4,6-diarylpyrimidine-5-carbonitriles as Potent and Selective A<sub>1</sub> Antagonists.
Val C, Rodríguez-García C, Prieto-Díaz R, Crespo A, Azuaje J, Carbajales C, Majellaro M, Díaz-Holguín A, Brea JM, Loza MI, Gioé-Gallo C, Contino M, Stefanachi A, García-Mera X, Estévez JC, Gutiérrez-de-Terán H, Sotelo E.
J Med Chem (2022) 65 : 2091 -2106
PubMed 35068155 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 6.94 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL369573 1 8.96
CHEMBL178584 1 7.95
CHEMBL259319 1 6.80
CHEMBL261185 1 6.39
CHEMBL406788 1 6.34
CHEMBL258834 1 6.25
CHEMBL3937015 1 5.86

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL369573 Ki = 1.1 nM 8.96
CHEMBL178584 Ki = 11.2 nM 7.95
CHEMBL259319 Ki = 159.0 nM 6.80
CHEMBL261185 Ki = 405.0 nM 6.39
CHEMBL406788 Ki = 459.0 nM 6.34
CHEMBL258834 Ki = 557.0 nM 6.25
CHEMBL3937015 Ki = 1369.0 nM 5.86