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Assay Detail

CHEMBL5045649

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-ATAD2 BD (981 to 1108 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) and biotinylated tetra-acetylated histone H4 peptides as substrate incubated for 30 mins by TR-FRET assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATPase family AAA domain-containing protein 2 (CHEMBL2150837)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent and Selective ATAD2 Bromodomain Inhibitor with Antiproliferative Activity in Breast Cancer Models.
Winter-Holt JJ, Bardelle C, Chiarparin E, Dale IL, Davey PRJ, Davies NL, Denz C, Fillery SM, Guérot CM, Han F, Hughes SJ, Kulkarni M, Liu Z, Milbradt A, Moss TA, Niu H, Patel J, Rabow AA, Schimpl M, Shi J, Sun D, Yang D, Guichard S.
J Med Chem (2022) 65 : 3306 -3331
PubMed 35133824 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.21 8.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5079885 1 8.20
CHEMBL5087319 1 7.80
CHEMBL5090369 1 7.70
CHEMBL5093862 1 7.70
CHEMBL5077167 1 7.50
CHEMBL5086059 1 7.40
CHEMBL5086260 1 7.10
CHEMBL5074983 1 6.70
CHEMBL5084768 1 6.60
CHEMBL5093167 1 6.40
CHEMBL5083025 1 6.40
CHEMBL5092181 1 6.40
CHEMBL5083653 1 5.80
CHEMBL5074478 1 5.50
CHEMBL5090538 1 5.50
CHEMBL5087156 1 5.30
CHEMBL5075463 1 5.20
CHEMBL5087174 1 4.90
CHEMBL5074753 1 4.70
CHEMBL5090410 1 4.70
CHEMBL5083337 1 4.60
CHEMBL5084439 1 4.50
CHEMBL5077001 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5079885 IC50 = 6.31 nM 8.20
CHEMBL5087319 IC50 = 15.85 nM 7.80
CHEMBL5090369 IC50 = 19.95 nM 7.70
CHEMBL5093862 IC50 = 19.95 nM 7.70
CHEMBL5077167 IC50 = 31.62 nM 7.50
CHEMBL5086059 IC50 = 39.81 nM 7.40
CHEMBL5086260 IC50 = 79.43 nM 7.10
CHEMBL5074983 IC50 = 199.53 nM 6.70
CHEMBL5084768 IC50 = 251.19 nM 6.60
CHEMBL5093167 IC50 = 398.11 nM 6.40
CHEMBL5083025 IC50 = 398.11 nM 6.40
CHEMBL5092181 IC50 = 398.11 nM 6.40
CHEMBL5083653 IC50 = 1584.89 nM 5.80
CHEMBL5074478 IC50 = 3162.28 nM 5.50
CHEMBL5090538 IC50 = 3162.28 nM 5.50
CHEMBL5087156 IC50 = 5011.87 nM 5.30
CHEMBL5075463 IC50 = 6309.57 nM 5.20
CHEMBL5087174 IC50 = 12589.25 nM 4.90
CHEMBL5074753 IC50 = 19952.62 nM 4.70
CHEMBL5090410 IC50 = 19952.62 nM 4.70
CHEMBL5083337 IC50 = 25118.86 nM 4.60
CHEMBL5084439 IC50 = 31622.78 nM 4.50
CHEMBL5077001 IC50 > 19952.62 nM -