Assay Detail
Binding
CHEMBL5047428
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of GST-fused human JAK3 (810 to 1100 residues) expressed in insect cells using ULight-JAK-1 Tyr1023 peptide as substrate preincubated for 30 mins followed by substrate addition and further incubated for 20 mins in presence of ATP by FRET assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Investigation of Janus Kinase (JAK) Inhibitors for Lung Delivery and the Importance of Aldehyde Oxidase Metabolism.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 31 | 8.13 | 9.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5086526 | — | — | 1 | 9.80 |
| CHEMBL5076189 | — | — | 1 | 9.70 |
| CHEMBL5084861 | — | — | 1 | 9.50 |
| CHEMBL5075556 | — | — | 1 | 9.30 |
| CHEMBL5084542 | — | — | 1 | 9.10 |
| CHEMBL5078647 | — | — | 1 | 9.10 |
| CHEMBL5078141 | — | — | 1 | 8.90 |
| CHEMBL5085176 | — | — | 1 | 8.80 |
| CHEMBL5079248 | — | — | 1 | 8.60 |
| CHEMBL5094089 | — | — | 1 | 8.50 |
| CHEMBL5075673 | — | — | 1 | 8.50 |
| CHEMBL5077126 | — | — | 1 | 8.50 |
| CHEMBL5074310 | — | — | 1 | 8.50 |
| CHEMBL5084340 | — | — | 1 | 8.30 |
| CHEMBL5082928 | — | — | 1 | 8.30 |
| CHEMBL5087255 | — | — | 1 | 8.30 |
| CHEMBL5076101 | — | — | 1 | 8.30 |
| CHEMBL5076068 | — | — | 1 | 8.20 |
| CHEMBL5084913 | — | — | 1 | 8.00 |
| CHEMBL5077653 | — | — | 1 | 8.00 |
| CHEMBL5075223 | — | — | 1 | 8.00 |
| CHEMBL5074436 | — | — | 1 | 7.70 |
| CHEMBL5079734 | — | — | 1 | 7.70 |
| CHEMBL5088804 | — | — | 1 | 7.40 |
| CHEMBL5084809 | — | — | 1 | 7.30 |
| CHEMBL5076598 | — | — | 1 | 7.30 |
| CHEMBL5089877 | — | — | 1 | 7.10 |
| CHEMBL5075565 | — | — | 1 | 7.00 |
| CHEMBL5072425 | — | — | 1 | 6.60 |
| CHEMBL5073925 | — | — | 1 | 6.30 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5086526 | — | IC50 | = | 0.1585 | nM | 9.80 |
| CHEMBL5076189 | — | IC50 | = | 0.1995 | nM | 9.70 |
| CHEMBL5084861 | — | IC50 | = | 0.3162 | nM | 9.50 |
| CHEMBL5075556 | — | IC50 | = | 0.5012 | nM | 9.30 |
| CHEMBL5084542 | — | IC50 | = | 0.7943 | nM | 9.10 |
| CHEMBL5078647 | — | IC50 | = | 0.7943 | nM | 9.10 |
| CHEMBL5078141 | — | IC50 | = | 1.259 | nM | 8.90 |
| CHEMBL5085176 | — | IC50 | = | 1.585 | nM | 8.80 |
| CHEMBL5079248 | — | IC50 | = | 2.512 | nM | 8.60 |
| CHEMBL5094089 | — | IC50 | = | 3.162 | nM | 8.50 |
| CHEMBL5075673 | — | IC50 | = | 3.162 | nM | 8.50 |
| CHEMBL5077126 | — | IC50 | = | 3.162 | nM | 8.50 |
| CHEMBL5074310 | — | IC50 | = | 3.162 | nM | 8.50 |
| CHEMBL5084340 | — | IC50 | = | 5.012 | nM | 8.30 |
| CHEMBL5082928 | — | IC50 | = | 5.012 | nM | 8.30 |
| CHEMBL5087255 | — | IC50 | = | 5.012 | nM | 8.30 |
| CHEMBL5076101 | — | IC50 | = | 5.012 | nM | 8.30 |
| CHEMBL5076068 | — | IC50 | = | 6.31 | nM | 8.20 |
| CHEMBL5084913 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL5077653 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL5075223 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL5074436 | — | IC50 | = | 19.95 | nM | 7.70 |
| CHEMBL5079734 | — | IC50 | = | 19.95 | nM | 7.70 |
| CHEMBL5088804 | — | IC50 | = | 39.81 | nM | 7.40 |
| CHEMBL5084809 | — | IC50 | = | 50.12 | nM | 7.30 |
| CHEMBL5076598 | — | IC50 | = | 50.12 | nM | 7.30 |
| CHEMBL5089877 | — | IC50 | = | 79.43 | nM | 7.10 |
| CHEMBL5075565 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL5072425 | — | IC50 | = | 251.19 | nM | 6.60 |
| CHEMBL5073925 | — | IC50 | = | 501.19 | nM | 6.30 |
| CHEMBL5076662 | — | IC50 | = | 3162.28 | nM | 5.50 |