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Assay Detail

CHEMBL5049075

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GCS using C8-ceramide and UDP-glucose as substrate incubated for 1 hr by RapidFire mass spectrometry
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ceramide glucosyltransferase (CHEMBL2063)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Brain-Penetrant Glucosylceramide Synthase Inhibitors with a Novel Pharmacophore.
Tanaka Y, Seto M, Kakegawa K, Takami K, Kikuchi F, Yamamoto T, Nakamura M, Daini M, Murakami M, Ohashi T, Kasahara T, Wang J, Ikeda Z, Wada Y, Puenner F, Fujii T, Inazuka M, Sato S, Suzaki T, Oak JH, Takai Y, Kohara H, Kimoto K, Oki H, Mikami S, Sasaki M, Tanaka Y.
J Med Chem (2022) 65 : 4270 -4290
PubMed 35188773 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 6.47 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5085509 1 7.82
CHEMBL5092141 1 7.77
CHEMBL5083428 1 7.72
CHEMBL5078078 1 7.51
CHEMBL5077442 1 7.40
CHEMBL5086168 1 7.35
CHEMBL5086523 1 7.31
CHEMBL5091974 1 7.31
CHEMBL5076612 1 7.01
CHEMBL5091003 1 6.77
CHEMBL5089984 1 6.20
CHEMBL5081829 1 6.16
CHEMBL5088946 1 6.00
CHEMBL5083924 1 5.89
CHEMBL5090560 1 5.72
CHEMBL5074066 1 5.62
CHEMBL5069925 1 5.50
CHEMBL5085654 1 5.36
CHEMBL5071935 1 5.36
CHEMBL5081824 1 5.31
CHEMBL5093384 1 4.77
CHEMBL5069574 1 -
CHEMBL5085627 1 -
CHEMBL5085407 1 -
CHEMBL5082608 1 -
CHEMBL5083606 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5085509 IC50 = 15.0 nM 7.82
CHEMBL5092141 IC50 = 17.0 nM 7.77
CHEMBL5083428 IC50 = 19.0 nM 7.72
CHEMBL5078078 IC50 = 31.0 nM 7.51
CHEMBL5077442 IC50 = 40.0 nM 7.40
CHEMBL5086168 IC50 = 45.0 nM 7.35
CHEMBL5086523 IC50 = 49.0 nM 7.31
CHEMBL5091974 IC50 = 49.0 nM 7.31
CHEMBL5076612 IC50 = 97.0 nM 7.01
CHEMBL5091003 IC50 = 170.0 nM 6.77
CHEMBL5089984 IC50 = 630.0 nM 6.20
CHEMBL5081829 IC50 = 700.0 nM 6.16
CHEMBL5088946 IC50 = 1000.0 nM 6.00
CHEMBL5083924 IC50 = 1300.0 nM 5.89
CHEMBL5090560 IC50 = 1900.0 nM 5.72
CHEMBL5074066 IC50 = 2400.0 nM 5.62
CHEMBL5069925 IC50 = 3200.0 nM 5.50
CHEMBL5085654 IC50 = 4400.0 nM 5.36
CHEMBL5071935 IC50 = 4400.0 nM 5.36
CHEMBL5081824 IC50 = 4900.0 nM 5.31
CHEMBL5093384 IC50 = 17000.0 nM 4.77
CHEMBL5069574 IC50 > 100000.0 nM -
CHEMBL5085627 IC50 > 100000.0 nM -
CHEMBL5085407 IC50 > 100000.0 nM -
CHEMBL5082608 IC50 > 100000.0 nM -
CHEMBL5083606 IC50 > 100000.0 nM -