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Assay Detail

CHEMBL5050587

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human TLR8 expressed in Drosophila S2 by TR-FRET assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Toll-like receptor 8 (CHEMBL5805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Optimization of a Fragment-like TLR8 Binding Screening Hit to an <i>In Vivo</i> Efficacious TLR7/8 Antagonist.
Betschart C, Faller M, Zink F, Hemmig R, Blank J, Vangrevelinghe E, Bourrel M, Glatthar R, Behnke D, Barker K, Heizmann A, Angst D, Nimsgern P, Jacquier S, Junt T, Zipfel G, Ruzzante G, Loetscher P, Limonta S, Hawtin S, Andre CB, Boulay T, Feifel R, Knoepfel T.
ACS Med Chem Lett (2022) 13 : 658 -664
PubMed 35450354 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.94 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5080083 1 8.70
CHEMBL5079910 1 8.62
CHEMBL5091113 1 8.52
CHEMBL5090067 1 8.44
CHEMBL5078689 1 8.33
CHEMBL5085458 1 8.09
CHEMBL5085289 1 8.08
CHEMBL5077828 1 7.85
CHEMBL5086681 1 7.66
CHEMBL5077018 1 7.64
CHEMBL5083541 1 7.48
CHEMBL5081397 1 7.41
CHEMBL5089903 1 7.29
CHEMBL5089636 1 7.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5080083 IC50 = 2.0 nM 8.70
CHEMBL5079910 IC50 = 2.4 nM 8.62
CHEMBL5091113 IC50 = 3.0 nM 8.52
CHEMBL5090067 IC50 = 3.6 nM 8.44
CHEMBL5078689 IC50 = 4.7 nM 8.33
CHEMBL5085458 IC50 = 8.1 nM 8.09
CHEMBL5085289 IC50 = 8.3 nM 8.08
CHEMBL5077828 IC50 = 14.0 nM 7.85
CHEMBL5086681 IC50 = 22.0 nM 7.66
CHEMBL5077018 IC50 = 23.0 nM 7.64
CHEMBL5083541 IC50 = 33.0 nM 7.48
CHEMBL5081397 IC50 = 39.0 nM 7.41
CHEMBL5089903 IC50 = 51.0 nM 7.29
CHEMBL5089636 IC50 = 100.0 nM 7.00