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Assay Detail

CHEMBL5053344

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant His-tagged human KIF18A (1 to 467 residues) expressed in baculovirus expression system assessed as inhibition of microtubule-stimulated ATPase activity preincubated for 15 mins followed by ATP addition and further incubated for 15 mins and measured after 40 mins by ADP-Glo reagent based assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kinesin-like protein KIF18A (CHEMBL4523403)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Heteroaryl amides useful as kif18a inhibitors
(2020)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.92 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5070813 1 7.89
CHEMBL5075318 1 7.82
CHEMBL5075286 1 7.57
CHEMBL5084317 1 7.51
CHEMBL5080126 1 7.32
CHEMBL5088011 1 7.26
CHEMBL5086169 1 7.15
CHEMBL5094470 1 7.10
CHEMBL5092054 1 7.06
CHEMBL5086799 1 7.04
CHEMBL5081899 1 6.98
CHEMBL5075334 1 6.91
CHEMBL5080318 1 6.76
CHEMBL5073613 1 6.69
CHEMBL5079025 1 6.66
CHEMBL5092540 1 6.64
CHEMBL5079508 1 6.58
CHEMBL5076230 1 6.57
CHEMBL5087469 1 6.45
CHEMBL5078599 1 6.41
CHEMBL5078022 1 6.40
CHEMBL5082926 1 6.34
CHEMBL5074779 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5070813 IC50 = 13.0 nM 7.89
CHEMBL5075318 IC50 = 15.0 nM 7.82
CHEMBL5075286 IC50 = 27.0 nM 7.57
CHEMBL5084317 IC50 = 31.0 nM 7.51
CHEMBL5080126 IC50 = 48.0 nM 7.32
CHEMBL5088011 IC50 = 55.0 nM 7.26
CHEMBL5086169 IC50 = 71.0 nM 7.15
CHEMBL5094470 IC50 = 80.0 nM 7.10
CHEMBL5092054 IC50 = 88.0 nM 7.06
CHEMBL5086799 IC50 = 91.0 nM 7.04
CHEMBL5081899 IC50 = 105.0 nM 6.98
CHEMBL5075334 IC50 = 122.0 nM 6.91
CHEMBL5080318 IC50 = 173.0 nM 6.76
CHEMBL5073613 IC50 = 206.0 nM 6.69
CHEMBL5079025 IC50 = 218.0 nM 6.66
CHEMBL5092540 IC50 = 230.0 nM 6.64
CHEMBL5079508 IC50 = 264.0 nM 6.58
CHEMBL5076230 IC50 = 272.0 nM 6.57
CHEMBL5087469 IC50 = 355.0 nM 6.45
CHEMBL5078599 IC50 = 394.0 nM 6.41
CHEMBL5078022 IC50 = 402.0 nM 6.40
CHEMBL5082926 IC50 = 453.0 nM 6.34
CHEMBL5074779 IC50 = 916.0 nM 6.04