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Assay Detail

CHEMBL5053639

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of fluorescent-labeled E2 from LBD of ERalpha (unknown origin) by TR-FRET assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Heterodimeric GW7604 Derivatives: Modification of the Pharmacological Profile by Additional Interactions at the Coactivator Binding Site.
Knox AK, Kalchschmid C, Schuster D, Gaggia F, Gust R.
J Med Chem (2021) 64 : 5766 -5786
PubMed 33904307 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.64 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL135 ESTRADIOL 4.0 1 9.48
CHEMBL5076984 1 8.89
CHEMBL5086315 1 8.85
CHEMBL5087008 1 8.84
CHEMBL5092721 1 8.81
CHEMBL5092334 1 8.79
CHEMBL5075366 1 8.65
CHEMBL489 AFIMOXIFENE 3.0 1 8.65
CHEMBL5091421 1 8.58
CHEMBL5085956 1 8.56
CHEMBL5088360 1 8.56
CHEMBL1358 FULVESTRANT 4.0 1 8.54
CHEMBL5094540 1 8.47
CHEMBL5094443 1 8.40
CHEMBL5071793 1 8.39
CHEMBL5083251 1 8.37
CHEMBL5094584 1 8.33
CHEMBL1091535 1 8.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL135 ESTRADIOL IC50 = 0.33 nM 9.48
CHEMBL5076984 IC50 = 1.28 nM 8.89
CHEMBL5086315 IC50 = 1.41 nM 8.85
CHEMBL5087008 IC50 = 1.45 nM 8.84
CHEMBL5092721 IC50 = 1.56 nM 8.81
CHEMBL5092334 IC50 = 1.62 nM 8.79
CHEMBL5075366 IC50 = 2.22 nM 8.65
CHEMBL489 AFIMOXIFENE IC50 = 2.24 nM 8.65
CHEMBL5091421 IC50 = 2.61 nM 8.58
CHEMBL5085956 IC50 = 2.75 nM 8.56
CHEMBL5088360 IC50 = 2.72 nM 8.56
CHEMBL1358 FULVESTRANT IC50 = 2.85 nM 8.54
CHEMBL5094540 IC50 = 3.41 nM 8.47
CHEMBL5094443 IC50 = 4.01 nM 8.40
CHEMBL5071793 IC50 = 4.11 nM 8.39
CHEMBL5083251 IC50 = 4.23 nM 8.37
CHEMBL5094584 IC50 = 4.63 nM 8.33
CHEMBL1091535 IC50 = 5.35 nM 8.27