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Assay Detail

CHEMBL5055814

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Binding
Antagonist activity at rat P2X7 receptor expressed in HEK293 cells assessed as inhibition of BzATP-induced calcium-flux measured after 60 mins by FLIPR analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL2496)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and Characterization of the Novel Rodent-Active and CNS-Penetrant P2X7 Receptor Antagonist Lu AF27139.
Hopper AT, Juhl M, Hornberg J, Badolo L, Kilburn JP, Thougaard A, Smagin G, Song D, Calice L, Menon V, Dale E, Zhang H, Cajina M, Nattini ME, Gandhi A, Grenon M, Jones K, Khayrullina T, Chandrasena G, Thomsen C, Zorn SH, Brodbeck R, Poda SB, Staal R, Möller T.
J Med Chem (2021) 64 : 4891 -4902
PubMed 33822617 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.04 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5086274 1 8.62
CHEMBL255787 1 8.40
CHEMBL3545108 AZD9056 2.0 1 8.36
CHEMBL2338352 1 8.33
CHEMBL1628690 1 8.21
CHEMBL437703 1 7.15
CHEMBL5075634 1 7.02
CHEMBL5498461 E133 1 6.70
CHEMBL3952549 1 6.58
CHEMBL1823817 CE-224535 2.0 1 6.33
CHEMBL377219 1 6.04
CHEMBL5087215 1 5.82
CHEMBL1222883 GSK1482160 1.0 1 5.72
CHEMBL1094153 1 5.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5086274 IC50 = 2.4 nM 8.62
CHEMBL255787 IC50 = 4.0 nM 8.40
CHEMBL3545108 AZD9056 IC50 = 4.4 nM 8.36
CHEMBL2338352 IC50 = 4.7 nM 8.33
CHEMBL1628690 IC50 = 6.1 nM 8.21
CHEMBL437703 IC50 = 71.0 nM 7.15
CHEMBL5075634 IC50 = 95.0 nM 7.02
CHEMBL5498461 E133 IC50 = 200.0 nM 6.70
CHEMBL3952549 IC50 = 260.0 nM 6.58
CHEMBL1823817 CE-224535 IC50 = 470.0 nM 6.33
CHEMBL377219 IC50 = 910.0 nM 6.04
CHEMBL5087215 IC50 = 1500.0 nM 5.82
CHEMBL1222883 GSK1482160 IC50 = 1900.0 nM 5.72
CHEMBL1094153 IC50 = 5500.0 nM 5.26