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Assay Detail

CHEMBL5055818

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-A-804598 from rat P2X7 receptor expressed in HEK293 cell membrane by in vitro binding assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL2496)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and Characterization of the Novel Rodent-Active and CNS-Penetrant P2X7 Receptor Antagonist Lu AF27139.
Hopper AT, Juhl M, Hornberg J, Badolo L, Kilburn JP, Thougaard A, Smagin G, Song D, Calice L, Menon V, Dale E, Zhang H, Cajina M, Nattini ME, Gandhi A, Grenon M, Jones K, Khayrullina T, Chandrasena G, Thomsen C, Zorn SH, Brodbeck R, Poda SB, Staal R, Möller T.
J Med Chem (2021) 64 : 4891 -4902
PubMed 33822617 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 6.95 8.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1628690 1 8.41
CHEMBL5086274 1 7.89
CHEMBL437703 1 7.80
CHEMBL255787 1 7.23
CHEMBL2338352 1 7.01
CHEMBL3545108 AZD9056 2.0 1 6.89
CHEMBL377219 1 6.70
CHEMBL1222883 GSK1482160 1.0 1 6.29
CHEMBL5498461 E133 1 6.00
CHEMBL1094153 1 5.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1628690 Ki = 3.9 nM 8.41
CHEMBL5086274 Ki = 13.0 nM 7.89
CHEMBL437703 Ki = 16.0 nM 7.80
CHEMBL255787 Ki = 59.0 nM 7.23
CHEMBL2338352 Ki = 98.0 nM 7.01
CHEMBL3545108 AZD9056 Ki = 130.0 nM 6.89
CHEMBL377219 Ki = 200.0 nM 6.70
CHEMBL1222883 GSK1482160 Ki = 510.0 nM 6.29
CHEMBL5498461 E133 Ki = 1000.0 nM 6.00
CHEMBL1094153 Ki = 4800.0 nM 5.32