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Assay Detail

CHEMBL5055910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human serum BChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition by Ellman's method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL1914)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Sustainable Drug Discovery of Multi-Target-Directed Ligands for Alzheimer's Disease.
Rossi M, Freschi M, de Camargo Nascente L, Salerno A, de Melo Viana Teixeira S, Nachon F, Chantegreil F, Soukup O, Prchal L, Malaguti M, Bergamini C, Bartolini M, Angeloni C, Hrelia S, Soares Romeiro LA, Bolognesi ML.
J Med Chem (2021) 64 : 4972 -4990
PubMed 33829779 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.91 10.45

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5080685 1 10.45
CHEMBL5092094 1 9.75
CHEMBL5078555 1 9.58
CHEMBL5084379 1 8.46
CHEMBL5073819 1 8.43
CHEMBL5082773 1 8.37
CHEMBL5091660 1 8.11
CHEMBL5087646 1 7.95
CHEMBL5072428 1 7.71
CHEMBL5088557 1 7.59
CHEMBL5088541 1 7.54
CHEMBL95 TACRINE 4.0 1 7.34
CHEMBL5077234 1 7.08
CHEMBL5078914 1 6.92
CHEMBL292314 1 6.30
CHEMBL1256415 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5080685 IC50 = 0.0352 nM 10.45
CHEMBL5092094 IC50 = 0.177 nM 9.75
CHEMBL5078555 IC50 = 0.265 nM 9.58
CHEMBL5084379 IC50 = 3.49 nM 8.46
CHEMBL5073819 IC50 = 3.74 nM 8.43
CHEMBL5082773 IC50 = 4.25 nM 8.37
CHEMBL5091660 IC50 = 7.72 nM 8.11
CHEMBL5087646 IC50 = 11.2 nM 7.95
CHEMBL5072428 IC50 = 19.4 nM 7.71
CHEMBL5088557 IC50 = 25.8 nM 7.59
CHEMBL5088541 IC50 = 29.2 nM 7.54
CHEMBL95 TACRINE IC50 = 45.8 nM 7.34
CHEMBL5077234 IC50 = 83.2 nM 7.08
CHEMBL5078914 IC50 = 120.0 nM 6.92
CHEMBL292314 IC50 = 505.0 nM 6.30
CHEMBL1256415 IC50 = 8860.0 nM 5.05