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Assay Detail

CHEMBL5096569

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST fused recombinant SIK2 in HEK293 cells assessed as effect on CRTC3 phosphorylation at Ser329 incubated for 1 hr in presence of ATP by autoradiography based immunoblotting analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase SIK2 (CHEMBL5699)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and Therapeutic Potential of NUAKs Inhibitors.
Faisal M, Kim JH, Yoo KH, Roh EJ, Hong SS, Lee SH.
J Med Chem (2021) 64 : 2 -25
PubMed 33356242 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.92 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5199998 1 7.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5199998 IC50 = 12.0 nM 7.92