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Assay Detail

CHEMBL5098435

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of RANKL/M-CSF-induced osteoclastogenesis in mouse RAW264.7 cells measured for 4 days by TRAP staining based assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis and Biological Evaluation of C-17-Amino-Substituted Pyrazole-Fused Betulinic Acid Derivatives as Novel Agents for Osteoarthritis Treatment.
Wang J, Wei W, Zhang X, Cao S, Hu B, Ye Y, Jiang M, Wang T, Zuo J, He S, Yang C.
J Med Chem (2021) 64 : 13676 -13692
PubMed 34491054 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.21 6.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5186869 1 6.07
CHEMBL5184893 1 6.07
CHEMBL5178636 1 5.98
CHEMBL5173721 1 5.73
CHEMBL5185421 1 5.44
CHEMBL5208353 1 5.16
CHEMBL5204621 1 5.06
CHEMBL5188983 1 5.06
CHEMBL5192399 1 4.89
CHEMBL5182318 1 4.83
CHEMBL5182336 1 4.76
CHEMBL5206216 1 4.68
CHEMBL5174287 1 4.67
CHEMBL269277 BETULINIC ACID 1.0 1 4.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5186869 IC50 = 850.0 nM 6.07
CHEMBL5184893 IC50 = 860.0 nM 6.07
CHEMBL5178636 IC50 = 1050.0 nM 5.98
CHEMBL5173721 IC50 = 1860.0 nM 5.73
CHEMBL5185421 IC50 = 3660.0 nM 5.44
CHEMBL5208353 IC50 = 7000.0 nM 5.16
CHEMBL5204621 IC50 = 8680.0 nM 5.06
CHEMBL5188983 IC50 = 8700.0 nM 5.06
CHEMBL5192399 IC50 = 12750.0 nM 4.89
CHEMBL5182318 IC50 = 14970.0 nM 4.83
CHEMBL5182336 IC50 = 17500.0 nM 4.76
CHEMBL5206216 IC50 = 20750.0 nM 4.68
CHEMBL5174287 IC50 = 21590.0 nM 4.67
CHEMBL269277 BETULINIC ACID IC50 = 32380.0 nM 4.49