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Assay Detail

CHEMBL5098448

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity against LPS-induced mouse RAW264.7 cells assessed as reduction in IL-1beta production incubated for 48 hrs by ELISA analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis and Biological Evaluation of C-17-Amino-Substituted Pyrazole-Fused Betulinic Acid Derivatives as Novel Agents for Osteoarthritis Treatment.
Wang J, Wei W, Zhang X, Cao S, Hu B, Ye Y, Jiang M, Wang T, Zuo J, He S, Yang C.
J Med Chem (2021) 64 : 13676 -13692
PubMed 34491054 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.82 5.63

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5173721 1 5.63
CHEMBL5185421 1 4.79
CHEMBL5188983 1 4.68
CHEMBL41286 DIACEREIN 3.0 1 4.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5173721 IC50 = 2320.0 nM 5.63
CHEMBL5185421 IC50 = 16100.0 nM 4.79
CHEMBL5188983 IC50 = 20720.0 nM 4.68
CHEMBL41286 DIACEREIN IC50 = 65410.0 nM 4.18