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Assay Detail

CHEMBL5099261

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CA200645 binding to NanoLuc-fused human adenosine A1 receptor expressed in HEK293 cells measured for 10 mins by NanoBRET competition binding assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A1 (CHEMBL226)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Structure-Activity Relationship Studies of Novel Adenosine A<sub>1</sub> Receptor-Selective Agonists.
Preti B, Suchankova A, Deganutti G, Leuenberger M, Barkan K, Manulak I, Huang X, Carvalho S, Ladds G, Lochner M.
J Med Chem (2022) 65 : 14864 -14890
PubMed 36270633 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 6.56 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5174052 1 7.55
CHEMBL5174429 1 7.43
CHEMBL5195714 1 7.39
CHEMBL5171042 1 7.30
CHEMBL5187330 1 7.26
CHEMBL5205907 1 7.19
CHEMBL5197358 1 7.17
CHEMBL5203632 1 7.05
CHEMBL5191989 1 6.86
CHEMBL5186757 1 6.84
CHEMBL5187478 1 6.67
CHEMBL5208245 1 6.63
CHEMBL5201013 1 6.61
CHEMBL464859 NECA 1 6.61
CHEMBL5199871 1 6.56
CHEMBL5172389 1 6.54
CHEMBL5184828 1 6.49
CHEMBL5170857 1 6.39
CHEMBL5171285 1 6.35
CHEMBL5188601 1 6.35
CHEMBL5186981 1 6.23
CHEMBL3771208 1 6.18
CHEMBL5179726 1 6.16
CHEMBL5206687 1 6.15
CHEMBL5209222 1 6.15
CHEMBL477 ADENOSINE 4.0 1 6.09
CHEMBL5182056 1 5.94
CHEMBL5183907 1 5.90
CHEMBL5196040 1 5.85
CHEMBL5171835 1 5.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5174052 Ki = 28.18 nM 7.55
CHEMBL5174429 Ki = 37.15 nM 7.43
CHEMBL5195714 Ki = 40.74 nM 7.39
CHEMBL5171042 Ki = 50.12 nM 7.30
CHEMBL5187330 Ki = 54.95 nM 7.26
CHEMBL5205907 Ki = 64.57 nM 7.19
CHEMBL5197358 Ki = 67.61 nM 7.17
CHEMBL5203632 Ki = 89.13 nM 7.05
CHEMBL5191989 Ki = 138.04 nM 6.86
CHEMBL5186757 Ki = 144.54 nM 6.84
CHEMBL5187478 Ki = 213.8 nM 6.67
CHEMBL5208245 Ki = 234.42 nM 6.63
CHEMBL5201013 Ki = 245.47 nM 6.61
CHEMBL464859 NECA Ki = 245.47 nM 6.61
CHEMBL5199871 Ki = 275.42 nM 6.56
CHEMBL5172389 Ki = 288.4 nM 6.54
CHEMBL5184828 Ki = 323.59 nM 6.49
CHEMBL5170857 Ki = 407.38 nM 6.39
CHEMBL5171285 Ki = 446.68 nM 6.35
CHEMBL5188601 Ki = 446.68 nM 6.35
CHEMBL5186981 Ki = 588.84 nM 6.23
CHEMBL3771208 Ki = 660.69 nM 6.18
CHEMBL5179726 Ki = 691.83 nM 6.16
CHEMBL5206687 Ki = 707.95 nM 6.15
CHEMBL5209222 Ki = 707.95 nM 6.15
CHEMBL477 ADENOSINE Ki = 812.83 nM 6.09
CHEMBL5182056 Ki = 1148.15 nM 5.94
CHEMBL5183907 Ki = 1258.93 nM 5.90
CHEMBL5196040 Ki = 1412.54 nM 5.85
CHEMBL5171835 Ki = 1698.24 nM 5.77
CHEMBL5184329 Ki = 2630.27 nM 5.58