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Assay Detail

CHEMBL5099271

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CA200645 binding to NanoLuc-fused human adenosine A1 receptor expressed in HEK293 cells assessed as kinetic dissociation constant by NanoBRET competition binding assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A1 (CHEMBL226)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Structure-Activity Relationship Studies of Novel Adenosine A<sub>1</sub> Receptor-Selective Agonists.
Preti B, Suchankova A, Deganutti G, Leuenberger M, Barkan K, Manulak I, Huang X, Carvalho S, Ladds G, Lochner M.
J Med Chem (2022) 65 : 14864 -14890
PubMed 36270633 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 27 6.75 7.81

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5205907 1 7.81
CHEMBL5174429 1 7.75
CHEMBL5195714 1 7.66
CHEMBL5171042 1 7.63
CHEMBL5174052 1 7.47
CHEMBL5197358 1 7.34
CHEMBL5187330 1 7.23
CHEMBL5186757 1 6.97
CHEMBL5208245 1 6.94
CHEMBL5203632 1 6.91
CHEMBL5201013 1 6.90
CHEMBL5187478 1 6.77
CHEMBL5199871 1 6.74
CHEMBL5191989 1 6.71
CHEMBL5184828 1 6.67
CHEMBL464859 NECA 1 6.63
CHEMBL477 ADENOSINE 4.0 1 6.53
CHEMBL5209222 1 6.48
CHEMBL5171285 1 6.44
CHEMBL3771208 1 6.30
CHEMBL5188601 1 6.23
CHEMBL5186981 1 6.22
CHEMBL5206687 1 6.13
CHEMBL5170857 1 6.04
CHEMBL5182056 1 6.02
CHEMBL5172389 1 5.94
CHEMBL5179726 1 5.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5205907 Kd = 15.49 nM 7.81
CHEMBL5174429 Kd = 17.78 nM 7.75
CHEMBL5195714 Kd = 21.88 nM 7.66
CHEMBL5171042 Kd = 23.44 nM 7.63
CHEMBL5174052 Kd = 33.88 nM 7.47
CHEMBL5197358 Kd = 45.71 nM 7.34
CHEMBL5187330 Kd = 58.88 nM 7.23
CHEMBL5186757 Kd = 107.15 nM 6.97
CHEMBL5208245 Kd = 114.82 nM 6.94
CHEMBL5203632 Kd = 123.03 nM 6.91
CHEMBL5201013 Kd = 125.89 nM 6.90
CHEMBL5187478 Kd = 169.82 nM 6.77
CHEMBL5199871 Kd = 181.97 nM 6.74
CHEMBL5191989 Kd = 194.98 nM 6.71
CHEMBL5184828 Kd = 213.8 nM 6.67
CHEMBL464859 NECA Kd = 234.42 nM 6.63
CHEMBL477 ADENOSINE Kd = 295.12 nM 6.53
CHEMBL5209222 Kd = 331.13 nM 6.48
CHEMBL5171285 Kd = 363.08 nM 6.44
CHEMBL3771208 Kd = 501.19 nM 6.30
CHEMBL5188601 Kd = 588.84 nM 6.23
CHEMBL5186981 Kd = 602.56 nM 6.22
CHEMBL5206687 Kd = 741.31 nM 6.13
CHEMBL5170857 Kd = 912.01 nM 6.04
CHEMBL5182056 Kd = 954.99 nM 6.02
CHEMBL5172389 Kd = 1148.15 nM 5.94
CHEMBL5179726 Kd = 1445.44 nM 5.84