Assay Detail
Binding
CHEMBL5111625
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant LIMK1 (330 to 637 residues) (unknown origin) expressed in baculovirus infected insect cells using CFL1 as substrate preincubated for 10 mins followed by substrate addition and measured after 60 mins in presence of ATP by RapidFire mass spectrometry
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Development and Characterization of Type I, Type II, and Type III LIM-Kinase Chemical Probes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.67 | 8.15 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5169387 | — | — | 1 | 8.15 |
| CHEMBL2141887 | — | — | 1 | 7.78 |
| CHEMBL4790618 | — | — | 1 | 7.08 |
| CHEMBL4779405 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5169387 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL2141887 | — | IC50 | = | 16.6 | nM | 7.78 |
| CHEMBL4790618 | — | IC50 | = | 83.8 | nM | 7.08 |
| CHEMBL4779405 | — | IC50 | > | 50000.0 | nM | - |