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Assay Detail

CHEMBL5111629

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Binding
Binding affinity to LIMK1 in human HeLa cells assessed as apparent dissociation constant incubated for 45 mins by kinobead based pulldown assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

LIM domain kinase 1 (CHEMBL3836)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and Characterization of Type I, Type II, and Type III LIM-Kinase Chemical Probes.
Hanke T, Mathea S, Woortman J, Salah E, Berger BT, Tumber A, Kashima R, Hata A, Kuster B, Müller S, Knapp S.
J Med Chem (2022) 65 : 13264 -13287
PubMed 36136092 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 3 6.72 7.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2141887 1 7.23
CHEMBL5169387 1 6.75
CHEMBL4790618 1 6.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2141887 Kd = 59.0 nM 7.23
CHEMBL5169387 Kd = 177.0 nM 6.75
CHEMBL4790618 Kd = 682.0 nM 6.17