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Assay Detail

CHEMBL5111700

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of his6 tagged LIMK1 (unknown origin) expressed in baculovirus-infected Sf9 cells measured after 1 hr in presence of ATP by fluorescence based analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

LIM domain kinase 1 (CHEMBL3836)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and Characterization of Type I, Type II, and Type III LIM-Kinase Chemical Probes.
Hanke T, Mathea S, Woortman J, Salah E, Berger BT, Tumber A, Kashima R, Hata A, Kuster B, Müller S, Knapp S.
J Med Chem (2022) 65 : 13264 -13287
PubMed 36136092 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.10 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL212948 DAMNACANTHAL 1 6.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL212948 DAMNACANTHAL IC50 = 800.0 nM 6.10