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Assay Detail

CHEMBL5115459

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PAK4 (unknown origin) incubated for 60 mins by time-resolved HTRF assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PAK 4 (CHEMBL4482)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological evaluation of novel pyrazolo [3,4-d]pyrimidine derivatives as potent PLK4 inhibitors for the treatment of TRIM37-amplified breast cancer.
Sun Y, Sun Y, Wang L, Wu T, Yin W, Wang J, Xue Y, Qin Q, Sun Y, Yang H, Zhao D, Cheng M.
Eur J Med Chem (2022) 238 : 114424 -114424
PubMed 35576702 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 6.06 7.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5209160 1 7.02
CHEMBL5188129 1 6.64
CHEMBL5186873 1 6.56
CHEMBL5185324 1 6.41
CHEMBL5200865 1 6.40
CHEMBL5199896 1 6.34
CHEMBL5171469 1 6.29
CHEMBL5175294 1 6.21
CHEMBL5176789 1 6.18
CHEMBL5171233 1 6.17
CHEMBL5176750 1 5.98
CHEMBL5208535 1 5.96
CHEMBL5187941 1 5.88
CHEMBL5205336 1 5.82
CHEMBL5185001 1 5.76
CHEMBL5179518 1 5.71
CHEMBL5181286 1 5.36
CHEMBL5192817 1 5.23
CHEMBL5173615 1 5.23
CHEMBL5194020 1 -
CHEMBL5169342 1 -
CHEMBL5180759 1 -
CHEMBL5198607 1 -
CHEMBL5175172 1 -
CHEMBL5187903 1 -
CHEMBL5198928 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5209160 IC50 = 96.1 nM 7.02
CHEMBL5188129 IC50 = 231.1 nM 6.64
CHEMBL5186873 IC50 = 273.4 nM 6.56
CHEMBL5185324 IC50 = 389.2 nM 6.41
CHEMBL5200865 IC50 = 395.1 nM 6.40
CHEMBL5199896 IC50 = 458.2 nM 6.34
CHEMBL5171469 IC50 = 515.2 nM 6.29
CHEMBL5175294 IC50 = 610.6 nM 6.21
CHEMBL5176789 IC50 = 654.8 nM 6.18
CHEMBL5171233 IC50 = 668.3 nM 6.17
CHEMBL5176750 IC50 = 1051.8 nM 5.98
CHEMBL5208535 IC50 = 1093.0 nM 5.96
CHEMBL5187941 IC50 = 1324.8 nM 5.88
CHEMBL5205336 IC50 = 1501.2 nM 5.82
CHEMBL5185001 IC50 = 1750.1 nM 5.76
CHEMBL5179518 IC50 = 1961.1 nM 5.71
CHEMBL5181286 IC50 = 4336.2 nM 5.36
CHEMBL5192817 IC50 = 5898.6 nM 5.23
CHEMBL5173615 IC50 = 5937.1 nM 5.23
CHEMBL5194020 IC50 - -
CHEMBL5169342 IC50 - -
CHEMBL5180759 IC50 - -
CHEMBL5198607 IC50 - -
CHEMBL5175172 IC50 - -
CHEMBL5187903 IC50 - -
CHEMBL5198928 IC50 - -