Assay Detail
Binding
CHEMBL5117307
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal His/SUMO tagged recombinant SARS-CoV2 main protease expressed in Escherichia coli BL21 (DE3) cells using DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-MetGlu-EDANS as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured after 30 mins by fluorescence based assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1ab (CHEMBL4523582) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
Publication
A multi-pronged evaluation of aldehyde-based tripeptidyl main protease inhibitors as SARS-CoV-2 antivirals.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.48 | 8.07 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5171771 | — | — | 1 | 8.07 |
| CHEMBL5183770 | — | — | 1 | 7.82 |
| CHEMBL5202077 | — | — | 1 | 7.48 |
| CHEMBL5203900 | — | — | 1 | 7.33 |
| CHEMBL5178763 | — | — | 1 | 7.22 |
| CHEMBL4852855 | — | — | 1 | 6.98 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5171771 | — | IC50 | = | 8.5 | nM | 8.07 |
| CHEMBL5183770 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL5202077 | — | IC50 | = | 33.0 | nM | 7.48 |
| CHEMBL5203900 | — | IC50 | = | 47.0 | nM | 7.33 |
| CHEMBL5178763 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL4852855 | — | IC50 | = | 105.0 | nM | 6.98 |