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Assay Detail

CHEMBL5118655

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FLAG and C-terminally Avi-tagged full length SMYD2 (unknown origin) expressed in Sf9 cells using SAM and histone H3 (1 to 29 residues) as peptide substrate preincubated for 30 mins followed by substrate addition by radiometric assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

N-lysine methyltransferase SMYD2 (CHEMBL2169716)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Protein Lysine Methyltransferase SMYD2: A Promising Small Molecule Target for Cancer Therapy.
Zheng Q, Zhang W, Rao GW.
J Med Chem (2022) 65 : 10119 -10132
PubMed 35914250 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.11 8.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5187983 1 8.41
CHEMBL5197760 1 7.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5187983 IC50 = 3.9 nM 8.41
CHEMBL5197760 IC50 = 16.0 nM 7.80