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Assay Detail

CHEMBL5120428

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R/T790M/C797S mutant (unknown origin) phosphorylation expressed in mouse BaF3 cells measured after 4 hrs by HTRF assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BaF3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel Allosteric EGFR L858R Inhibitors for the Treatment of Non-Small-Cell Lung Cancer as a Single Agent or in Combination with Osimertinib.
Obst-Sander U, Ricci A, Kuhn B, Friess T, Koldewey P, Kuglstatter A, Hewings D, Goergler A, Steiner S, Rueher D, Imhoff MP, Raschetti N, Marty HP, Dietzig A, Rynn C, Ehler A, Burger D, Kornacker M, Schaffland JP, Herting F, Pao W, Bischoff JR, Martoglio B, Alice Nagel Y, Jaeschke G.
J Med Chem (2022) 65 : 13052 -13073
PubMed 36178776 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 7.97 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5177023 1 8.70
CHEMBL5184760 1 8.52
CHEMBL5189574 1 8.52
CHEMBL5183324 1 8.40
CHEMBL5184539 1 8.40
CHEMBL5173979 1 8.40
CHEMBL5202945 1 8.40
CHEMBL5180839 1 8.30
CHEMBL5207479 1 8.30
CHEMBL4214567 1 8.22
CHEMBL5208056 1 8.22
CHEMBL5193772 1 8.22
CHEMBL5193594 1 8.15
CHEMBL5180289 1 8.15
CHEMBL5189114 1 8.15
CHEMBL5187250 1 8.15
CHEMBL5172668 1 8.15
CHEMBL5170519 1 8.00
CHEMBL4443062 1 8.00
CHEMBL5203543 1 7.96
CHEMBL5198433 1 7.80
CHEMBL5203388 1 7.80
CHEMBL5207152 1 7.77
CHEMBL5173986 1 7.70
CHEMBL5201725 1 7.66
CHEMBL5195669 1 7.64
CHEMBL5170278 1 6.94
CHEMBL5171139 1 6.34
CHEMBL5208369 1 6.03
CHEMBL5205834 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5177023 IC50 = 2.0 nM 8.70
CHEMBL5184760 IC50 = 3.0 nM 8.52
CHEMBL5189574 IC50 = 3.0 nM 8.52
CHEMBL5183324 IC50 = 4.0 nM 8.40
CHEMBL5184539 IC50 = 4.0 nM 8.40
CHEMBL5173979 IC50 = 4.0 nM 8.40
CHEMBL5202945 IC50 = 4.0 nM 8.40
CHEMBL5180839 IC50 = 5.0 nM 8.30
CHEMBL5207479 IC50 = 5.0 nM 8.30
CHEMBL4214567 IC50 = 6.0 nM 8.22
CHEMBL5208056 IC50 = 6.0 nM 8.22
CHEMBL5193772 IC50 = 6.0 nM 8.22
CHEMBL5193594 IC50 = 7.0 nM 8.15
CHEMBL5180289 IC50 = 7.0 nM 8.15
CHEMBL5189114 IC50 = 7.0 nM 8.15
CHEMBL5187250 IC50 = 7.0 nM 8.15
CHEMBL5172668 IC50 = 7.0 nM 8.15
CHEMBL5170519 IC50 = 10.0 nM 8.00
CHEMBL4443062 IC50 = 10.0 nM 8.00
CHEMBL5203543 IC50 = 11.0 nM 7.96
CHEMBL5198433 IC50 = 16.0 nM 7.80
CHEMBL5203388 IC50 = 16.0 nM 7.80
CHEMBL5207152 IC50 = 17.0 nM 7.77
CHEMBL5173986 IC50 = 20.0 nM 7.70
CHEMBL5201725 IC50 = 22.0 nM 7.66
CHEMBL5195669 IC50 = 23.0 nM 7.64
CHEMBL5170278 IC50 = 116.0 nM 6.94
CHEMBL5171139 IC50 = 460.0 nM 6.34
CHEMBL5208369 IC50 = 938.0 nM 6.03
CHEMBL5205834 IC50 > 10000.0 nM -