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Assay Detail

CHEMBL5120693

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIF-1alpha (unknown origin) transcriptional activity expressed in HEK293T cells co-transfected with renilla and HRE-firelfly luciferase incubated for 24 hrs by dual luciferase reporter gene assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hypoxia-inducible factor 1-alpha (CHEMBL4261)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Benzo[<i>d</i>]isoxazole Derivatives as Hypoxia-Inducible Factor (HIF)-1α Inhibitors.
Xue Z, Li H, Xie W, Xu Y, Zhou L, Qu ZB.
ACS Med Chem Lett (2022) 13 : 1864 -1869
PubMed 36518694 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.34 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5205360 1 7.62
CHEMBL5184354 1 7.62
CHEMBL5208153 1 7.40
CHEMBL5194403 1 7.39
CHEMBL5197796 1 7.38
CHEMBL5192165 1 7.12
CHEMBL5188937 1 6.77
CHEMBL5174696 1 6.70
CHEMBL5202378 1 6.60
CHEMBL5178011 1 6.51
CHEMBL5177951 1 6.32
CHEMBL5209002 1 6.19
CHEMBL5190853 1 6.19
CHEMBL5205347 1 6.09
CHEMBL5189187 1 6.03
CHEMBL5186754 1 5.77
CHEMBL1078237 1 5.64
CHEMBL427092 1 5.58
CHEMBL5199345 1 5.27
CHEMBL5207932 1 5.26
CHEMBL5188651 1 5.13
CHEMBL5188998 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5205360 IC50 = 24.0 nM 7.62
CHEMBL5184354 IC50 = 24.0 nM 7.62
CHEMBL5208153 IC50 = 40.0 nM 7.40
CHEMBL5194403 IC50 = 41.0 nM 7.39
CHEMBL5197796 IC50 = 42.0 nM 7.38
CHEMBL5192165 IC50 = 75.0 nM 7.12
CHEMBL5188937 IC50 = 170.0 nM 6.77
CHEMBL5174696 IC50 = 200.0 nM 6.70
CHEMBL5202378 IC50 = 250.0 nM 6.60
CHEMBL5178011 IC50 = 310.0 nM 6.51
CHEMBL5177951 IC50 = 480.0 nM 6.32
CHEMBL5209002 IC50 = 640.0 nM 6.19
CHEMBL5190853 IC50 = 640.0 nM 6.19
CHEMBL5205347 IC50 = 810.0 nM 6.09
CHEMBL5189187 IC50 = 940.0 nM 6.03
CHEMBL5186754 IC50 = 1700.0 nM 5.77
CHEMBL1078237 IC50 = 2300.0 nM 5.64
CHEMBL427092 IC50 = 2600.0 nM 5.58
CHEMBL5199345 IC50 = 5400.0 nM 5.27
CHEMBL5207932 IC50 = 5500.0 nM 5.26
CHEMBL5188651 IC50 = 7400.0 nM 5.13
CHEMBL5188998 IC50 = 10000.0 nM 5.00