Assay Detail
Binding
CHEMBL5120707
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human N-terminal GST-tagged EGFR (669 to 1210 residues) (unknown origin) L858R/T790M mutant expressed in baculovirus infected Sf21 insect cells using AQT0734 as substrate measured up to 240 mins by fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf21 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Structural Basis for Inhibition of Mutant EGFR with Lazertinib (YH25448).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 6.90 | 7.47 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4558324 | LAZERTINIB | 4.0 | 1 | 7.47 |
| CHEMBL4098072 | — | — | 1 | 6.65 |
| CHEMBL3353410 | OSIMERTINIB | 4.0 | 1 | 6.59 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4558324 | LAZERTINIB | Ki | = | 34.0 | nM | 7.47 |
| CHEMBL4098072 | — | Ki | = | 224.0 | nM | 6.65 |
| CHEMBL3353410 | OSIMERTINIB | Ki | = | 256.0 | nM | 6.59 |