Assay Detail
Binding
CHEMBL5122320
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant BRD4-BD2 expressed in Escherichia coli using C-Terminal-biotinylated histone H4 peptide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by microplate reader method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Targeting Bromodomain-Selective Inhibitors of BET Proteins in Drug Discovery and Development.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 6.69 | 6.69 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL222541 | — | — | 1 | 6.69 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL222541 | — | IC50 | = | 204.0 | nM | 6.69 |