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Assay Detail

CHEMBL5124463

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay
32
Total Activities
32
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Silencing Tautomerization to Isolate Unstable Physalins from <i>Physalis minima</i>.
Wu JP, Li LY, Li JR, Yu M, Zhao J, Xu QM, Gu YC, Zhang T, Zou ZM.
J Nat Prod (2022) 85 : 1522 -1539
PubMed 35608269 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 4.75 5.54
Inhibition 9 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5200787 1 5.54
CHEMBL5172878 1 5.51
CHEMBL5199906 1 5.44
CHEMBL5171523 1 5.19
CHEMBL504910 1 5.10
CHEMBL5208941 1 5.05
CHEMBL5182035 1 5.02
CHEMBL5190947 1 5.02
CHEMBL5205019 1 4.87
CHEMBL1835434 PHYSALIN G 1 4.87
CHEMBL5190130 1 4.83
CHEMBL5200537 1 4.76
CHEMBL5181567 1 4.62
CHEMBL5184046 1 4.59
CHEMBL5170466 1 4.55
CHEMBL5207644 1 4.49
CHEMBL1956111 1 4.37
CHEMBL5203759 1 4.37
CHEMBL5195529 1 4.36
CHEMBL4851096 1 4.30
CHEMBL5180180 1 4.25
CHEMBL5181057 1 4.12
CHEMBL5176322 1 4.10
CHEMBL5198674 1 -
CHEMBL5196803 1 -
CHEMBL5177559 1 -
CHEMBL5181890 1 -
CHEMBL5177640 1 -
CHEMBL504404 PHYSALIN B 1 -
CHEMBL5173888 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5200787 IC50 = 2900.0 nM 5.54
CHEMBL5172878 IC50 = 3100.0 nM 5.51
CHEMBL5199906 IC50 = 3600.0 nM 5.44
CHEMBL5171523 IC50 = 6400.0 nM 5.19
CHEMBL504910 IC50 = 7900.0 nM 5.10
CHEMBL5208941 IC50 = 9000.0 nM 5.05
CHEMBL5182035 IC50 = 9500.0 nM 5.02
CHEMBL5190947 IC50 = 9500.0 nM 5.02
CHEMBL5205019 IC50 = 13600.0 nM 4.87
CHEMBL1835434 PHYSALIN G IC50 = 13500.0 nM 4.87
CHEMBL5190130 IC50 = 14900.0 nM 4.83
CHEMBL5200537 IC50 = 17500.0 nM 4.76
CHEMBL5181567 IC50 = 23800.0 nM 4.62
CHEMBL5184046 IC50 = 25600.0 nM 4.59
CHEMBL5170466 IC50 = 28000.0 nM 4.55
CHEMBL5207644 IC50 = 32300.0 nM 4.49
CHEMBL1956111 IC50 = 42800.0 nM 4.37
CHEMBL5203759 IC50 = 43200.0 nM 4.37
CHEMBL5195529 IC50 = 43800.0 nM 4.36
CHEMBL4851096 IC50 = 49800.0 nM 4.30
CHEMBL5180180 IC50 = 56600.0 nM 4.25
CHEMBL5181057 IC50 = 75300.0 nM 4.12
CHEMBL5176322 IC50 = 78700.0 nM 4.10
CHEMBL5198674 Inhibition < 50.0 % -
CHEMBL5196803 Inhibition < 50.0 % -
CHEMBL5177559 Inhibition < 50.0 % -
CHEMBL5181890 Inhibition < 50.0 % -
CHEMBL5177640 Inhibition < 50.0 % -
CHEMBL504404 PHYSALIN B Inhibition < 50.0 % -
CHEMBL5173888 Inhibition < 50.0 % -
CHEMBL5175225 Inhibition < 50.0 % -
CHEMBL5200872 Inhibition < 50.0 % -