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Assay Detail

CHEMBL5128200

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BTK (unknown origin) transfected in HEK293T cells assessed as reduction in BTK phosphorylation preincubated for 30 mins followed by ATP addition and measured after 20 mins by Western blot analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Recent development of BTK-based dual inhibitors in the treatment of cancers.
Ran F, Liu Y, Xu Z, Meng C, Yang D, Qian J, Deng X, Zhang Y, Ling Y.
Eur J Med Chem (2022) 233 : 114232 -114232
PubMed 35247756 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.03 8.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4075449 1 8.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4075449 IC50 = 9.4 nM 8.03