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Assay Detail

CHEMBL5128686

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCG2 (unknown origin) expressed in human HEK293-A cells membrane vesicles assessed inhibition of ABCG2-mediated urate transport activity by rapid filtration technique
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293-A
Subcellular Membrane vesicle
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Targeting breast cancer resistance protein (BCRP/ABCG2): Functional inhibitors and expression modulators.
Zattoni IF, Delabio LC, Dutra JP, Kita DH, Scheiffer G, Hembecker M, Pereira GDS, Moure VR, Valdameri G.
Eur J Med Chem (2022) 237 : 114346 -114346
PubMed 35483322 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.95 7.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1164729 FEBUXOSTAT 4.0 1 7.57
CHEMBL1078685 TOPIROXOSTAT 2.0 1 6.75
CHEMBL388590 BENZBROMARONE 4.0 1 6.70
CHEMBL35 FUROSEMIDE 4.0 1 5.50
CHEMBL1487 ATORVASTATIN 4.0 1 5.37
CHEMBL672 FENOFIBRATE 4.0 1 5.29
CHEMBL191 LOSARTAN 4.0 1 4.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1164729 FEBUXOSTAT IC50 = 27.0 nM 7.57
CHEMBL1078685 TOPIROXOSTAT IC50 = 180.0 nM 6.75
CHEMBL388590 BENZBROMARONE IC50 = 200.0 nM 6.70
CHEMBL35 FUROSEMIDE IC50 = 3200.0 nM 5.50
CHEMBL1487 ATORVASTATIN IC50 = 4300.0 nM 5.37
CHEMBL672 FENOFIBRATE IC50 = 5100.0 nM 5.29
CHEMBL191 LOSARTAN IC50 = 35000.0 nM 4.46