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Assay Detail

CHEMBL5131017

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Modulation of homozygous F508del/F508del CFTR mutant in HBE cells incubated for 18 to 24 hrs by trans-epithelial current clamp assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HBE
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cystic fibrosis transmembrane conductance regulator (CHEMBL4051)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Substituted Cyclopropyl Compounds as CFTR Modulators for Treating Cystic Fibrosis.
Sabnis RW.
ACS Med Chem Lett (2022) 13 : 1552 -1553
PubMed 36262401 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 9.18 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5178232 1 9.40
CHEMBL5193680 1 9.29
CHEMBL5178488 1 9.18
CHEMBL5202275 1 9.10
CHEMBL5186971 1 9.08
CHEMBL5171054 1 9.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5178232 EC50 = 0.4 nM 9.40
CHEMBL5193680 EC50 = 0.51 nM 9.29
CHEMBL5178488 EC50 = 0.66 nM 9.18
CHEMBL5202275 EC50 = 0.8 nM 9.10
CHEMBL5186971 EC50 = 0.84 nM 9.08
CHEMBL5171054 EC50 = 0.9 nM 9.05