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Assay Detail

CHEMBL5134928

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human P2X2 receptor expressed in human 1321N1 cells assessed as reduction in intracellular Ca2+ influx incubated for 30 mins by Fura-2 AM based fluorescence assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type 1321N1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 2 (CHEMBL2531)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of adamantane-1-carbonyl thiourea derivatives as potent and selective inhibitors of h-P2X4 and h-P2X7 receptors: An Emerging therapeutic tool for treatment of inflammation and neurological disorders.
Mahmood A, Ali Shah SJ, Iqbal J.
Eur J Med Chem (2022) 231 : 114162 -114162
PubMed 35123298 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.60 6.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5200337 1 6.13
CHEMBL5187239 1 6.05
CHEMBL5174645 1 5.97
CHEMBL1615626 1 5.85
CHEMBL5183204 1 5.64
CHEMBL5175938 1 5.34
CHEMBL5172961 1 5.31
CHEMBL5186040 1 5.28
CHEMBL265502 SURAMIN 3.0 1 4.87
CHEMBL1532400 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5200337 IC50 = 732.0 nM 6.13
CHEMBL5187239 IC50 = 890.0 nM 6.05
CHEMBL5174645 IC50 = 1061.0 nM 5.97
CHEMBL1615626 IC50 = 1400.0 nM 5.85
CHEMBL5183204 IC50 = 2306.0 nM 5.64
CHEMBL5175938 IC50 = 4576.0 nM 5.34
CHEMBL5172961 IC50 = 4852.0 nM 5.31
CHEMBL5186040 IC50 = 5242.0 nM 5.28
CHEMBL265502 SURAMIN IC50 = 13450.0 nM 4.87
CHEMBL1532400 IC50 - -