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Assay Detail

CHEMBL5135397

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human lysosomal alpha glucosidase assessed as release of p-nitrophenol using p-nitrophenyl glycoside as substrate by spectrometric assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysosomal alpha-glucosidase (CHEMBL2608)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and glycosidase inhibition of C-4 branched LAB and DAB derivatives.
Wang JZ, Cheng B, Kato A, Kise M, Shimadate Y, Jia YM, Li YX, Fleet GWJ, Yu CY.
Eur J Med Chem (2022) 233 : 114230 -114230
PubMed 35255314 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.23 6.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5189075 1 6.20
CHEMBL5198563 1 5.62
CHEMBL5192700 1 5.43
CHEMBL2177689 1 5.25
CHEMBL5090583 1 4.80
CHEMBL5207259 1 4.10
CHEMBL406973 1 -
CHEMBL5202712 1 -
CHEMBL80254 1 -
CHEMBL5188686 1 -
CHEMBL5183794 1 -
CHEMBL5186198 1 -
CHEMBL5191437 1 -
CHEMBL5203897 1 -
CHEMBL5195202 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5189075 IC50 = 630.0 nM 6.20
CHEMBL5198563 IC50 = 2400.0 nM 5.62
CHEMBL5192700 IC50 = 3700.0 nM 5.43
CHEMBL2177689 IC50 = 5600.0 nM 5.25
CHEMBL5090583 IC50 = 16000.0 nM 4.80
CHEMBL5207259 IC50 = 79000.0 nM 4.10
CHEMBL406973 IC50 - -
CHEMBL5202712 IC50 = 601000.0 nM -
CHEMBL80254 IC50 - -
CHEMBL5188686 IC50 = 130000.0 nM -
CHEMBL5183794 IC50 = 491000.0 nM -
CHEMBL5186198 IC50 = 376000.0 nM -
CHEMBL5191437 IC50 = 714000.0 nM -
CHEMBL5203897 IC50 = 372000.0 nM -
CHEMBL5195202 IC50 = 776000.0 nM -