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Assay Detail

CHEMBL5135910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV-2 nsp13 helicase-associated activity using 5'- AGT CTT CTC CTG GTG CTC GAA CAG TGA CCy3-3', 5'- BHQ-2-GTC ACT GTT CGA GCA CCA CCT CTT CTG A-3' DNA as substrate preincubated for 10 mins followed by substrate addition and measured after 15 mins in the presence of ATP by fluorescent-based helicase unwind assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Discovery of 2-Phenylquinolines with Broad-Spectrum Anti-coronavirus Activity.
Nizi MG, Persoons L, Corona A, Felicetti T, Cernicchi G, Massari S, Manfroni G, Vangeel L, Barreca ML, Esposito F, Jochmans D, Milia J, Cecchetti V, Schols D, Neyts J, Tramontano E, Sabatini S, De Jonghe S, Tabarrini O.
ACS Med Chem Lett (2022) 13 : 855 -864
PubMed 35571875 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.52 7.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1595621 1 7.34
CHEMBL4175014 1 6.38
CHEMBL5181347 1 5.85
CHEMBL5173495 1 -
CHEMBL5209332 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1595621 IC50 = 46.0 nM 7.34
CHEMBL4175014 IC50 = 420.0 nM 6.38
CHEMBL5181347 IC50 = 1410.0 nM 5.85
CHEMBL5173495 IC50 > 30000.0 nM -
CHEMBL5209332 IC50 > 30000.0 nM -