Assay Detail
Binding
CHEMBL5136535
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of GCS in human A-375 cells using C6-ceramide and UDP-glucose as substrate preincubated for 30 mins followed by substrate addition measured after 20 hrs by UDP-Glo glucosylceramide synthase based luminometer analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | A-375 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Modified Benzofuran-carboxamide Compounds as Glucosylceramide Synthase Inhibitors for Treating Diseases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 6 | 10.51 | 10.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5182897 | — | — | 1 | 10.70 |
| CHEMBL5170109 | — | — | 1 | 10.68 |
| CHEMBL5191157 | — | — | 1 | 10.64 |
| CHEMBL5177098 | — | — | 1 | 10.36 |
| CHEMBL5195638 | — | — | 1 | 10.36 |
| CHEMBL5197108 | — | — | 1 | 10.33 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5182897 | — | EC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5170109 | — | EC50 | = | 0.021 | nM | 10.68 |
| CHEMBL5191157 | — | EC50 | = | 0.023 | nM | 10.64 |
| CHEMBL5177098 | — | EC50 | = | 0.044 | nM | 10.36 |
| CHEMBL5195638 | — | EC50 | = | 0.044 | nM | 10.36 |
| CHEMBL5197108 | — | EC50 | = | 0.047 | nM | 10.33 |