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Assay Detail

CHEMBL5136648

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant his tagged SARS CoV-2 main protease expressed in Escherichia coli BL21 using Boc-Abu-Tle-Leu-Gln-AMC37 as fluorogenic substrate pre-incubated for 5 mins followed by substrate addition measured after 60 mins by FRET based assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Small-Molecule Thioesters as SARS-CoV-2 Main Protease Inhibitors: Enzyme Inhibition, Structure-Activity Relationships, Antiviral Activity, and X-ray Structure Determination.
Pillaiyar T, Flury P, Krüger N, Su H, Schäkel L, Barbosa Da Silva E, Eppler O, Kronenberger T, Nie T, Luedtke S, Rocha C, Sylvester K, Petry MRI, McKerrow JH, Poso A, Pöhlmann S, Gütschow M, O'Donoghue AJ, Xu Y, Müller CE, Laufer SA.
J Med Chem (2022) 65 : 9376 -9395
PubMed 35709506 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 6.89 7.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5169323 1 7.94
CHEMBL5186338 1 7.86
CHEMBL5208709 1 7.84
CHEMBL5204680 1 7.81
CHEMBL5177782 1 7.74
CHEMBL5184362 1 7.69
CHEMBL4855437 1 7.46
CHEMBL5188939 1 7.26
CHEMBL5189565 1 7.22
CHEMBL5173920 1 7.14
CHEMBL5207081 1 7.06
CHEMBL5197891 1 7.05
CHEMBL5204452 1 7.04
CHEMBL5192654 1 6.93
CHEMBL5178568 1 6.90
CHEMBL5204130 1 6.84
CHEMBL5204064 1 6.83
CHEMBL5199191 1 6.80
CHEMBL5186708 1 6.78
CHEMBL5181137 1 6.72
CHEMBL5192027 1 6.63
CHEMBL5207392 1 6.60
CHEMBL5194755 1 6.59
CHEMBL5202104 1 6.55
CHEMBL5169680 1 6.54
CHEMBL5189863 1 6.50
CHEMBL5199531 1 6.46
CHEMBL5191017 1 6.34
CHEMBL5174547 1 6.32
CHEMBL5178465 1 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5169323 IC50 = 11.4 nM 7.94
CHEMBL5186338 IC50 = 13.7 nM 7.86
CHEMBL5208709 IC50 = 14.4 nM 7.84
CHEMBL5204680 IC50 = 15.5 nM 7.81
CHEMBL5177782 IC50 = 18.1 nM 7.74
CHEMBL5184362 IC50 = 20.6 nM 7.69
CHEMBL4855437 IC50 = 34.4 nM 7.46
CHEMBL5188939 IC50 = 54.7 nM 7.26
CHEMBL5189565 IC50 = 60.0 nM 7.22
CHEMBL5173920 IC50 = 72.3 nM 7.14
CHEMBL5207081 IC50 = 87.6 nM 7.06
CHEMBL5197891 IC50 = 88.9 nM 7.05
CHEMBL5204452 IC50 = 91.4 nM 7.04
CHEMBL5192654 IC50 = 117.0 nM 6.93
CHEMBL5178568 IC50 = 125.0 nM 6.90
CHEMBL5204130 IC50 = 143.0 nM 6.84
CHEMBL5204064 IC50 = 147.0 nM 6.83
CHEMBL5199191 IC50 = 159.0 nM 6.80
CHEMBL5186708 IC50 = 166.0 nM 6.78
CHEMBL5181137 IC50 = 190.0 nM 6.72
CHEMBL5192027 IC50 = 235.0 nM 6.63
CHEMBL5207392 IC50 = 250.0 nM 6.60
CHEMBL5194755 IC50 = 255.0 nM 6.59
CHEMBL5202104 IC50 = 281.0 nM 6.55
CHEMBL5169680 IC50 = 288.0 nM 6.54
CHEMBL5189863 IC50 = 316.0 nM 6.50
CHEMBL5199531 IC50 = 349.0 nM 6.46
CHEMBL5191017 IC50 = 454.0 nM 6.34
CHEMBL5174547 IC50 = 481.0 nM 6.32
CHEMBL5178465 IC50 = 551.0 nM 6.26
CHEMBL5184334 IC50 = 624.0 nM 6.21
CHEMBL5207613 IC50 = 1220.0 nM 5.91
CHEMBL5192248 IC50 = 2030.0 nM 5.69