Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5136650

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant his tagged SARS CoV-2 main protease expressed in Escherichia coli BL21 assessed as inhibition constant using Boc-Abu-Tle-Leu-Gln-AMC37 as fluorogenic substrate pre-incubated for 5 mins followed by substrate addition measured after 60 mins by FRET based assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Small-Molecule Thioesters as SARS-CoV-2 Main Protease Inhibitors: Enzyme Inhibition, Structure-Activity Relationships, Antiviral Activity, and X-ray Structure Determination.
Pillaiyar T, Flury P, Krüger N, Su H, Schäkel L, Barbosa Da Silva E, Eppler O, Kronenberger T, Nie T, Luedtke S, Rocha C, Sylvester K, Petry MRI, McKerrow JH, Poso A, Pöhlmann S, Gütschow M, O'Donoghue AJ, Xu Y, Müller CE, Laufer SA.
J Med Chem (2022) 65 : 9376 -9395
PubMed 35709506 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 29 7.07 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5194755 1 7.89
CHEMBL5169323 1 7.85
CHEMBL5178465 1 7.68
CHEMBL5191017 1 7.68
CHEMBL5207081 1 7.48
CHEMBL5207392 1 7.46
CHEMBL5189863 1 7.45
CHEMBL5178568 1 7.38
CHEMBL5204452 1 7.37
CHEMBL5197891 1 7.29
CHEMBL5192654 1 7.14
CHEMBL5204130 1 7.13
CHEMBL5202104 1 7.11
CHEMBL5204064 1 7.07
CHEMBL5207613 1 6.92
CHEMBL5181137 1 6.79
CHEMBL5188939 1 6.73
CHEMBL5199191 1 6.60
CHEMBL5199531 1 6.53
CHEMBL5173920 1 6.41
CHEMBL5192027 1 6.22
CHEMBL5186708 1 6.21
CHEMBL5192248 1 6.11
CHEMBL5184362 1 -
CHEMBL5208709 1 -
CHEMBL5177782 1 -
CHEMBL5174547 1 -
CHEMBL5189565 1 -
CHEMBL5184334 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5194755 Ki = 13.0 nM 7.89
CHEMBL5169323 Ki = 14.1 nM 7.85
CHEMBL5178465 Ki = 21.0 nM 7.68
CHEMBL5191017 Ki = 21.1 nM 7.68
CHEMBL5207081 Ki = 33.2 nM 7.48
CHEMBL5207392 Ki = 34.4 nM 7.46
CHEMBL5189863 Ki = 35.7 nM 7.45
CHEMBL5178568 Ki = 42.0 nM 7.38
CHEMBL5204452 Ki = 43.0 nM 7.37
CHEMBL5197891 Ki = 51.0 nM 7.29
CHEMBL5192654 Ki = 72.6 nM 7.14
CHEMBL5204130 Ki = 74.4 nM 7.13
CHEMBL5202104 Ki = 77.3 nM 7.11
CHEMBL5204064 Ki = 84.4 nM 7.07
CHEMBL5207613 Ki = 119.0 nM 6.92
CHEMBL5181137 Ki = 161.0 nM 6.79
CHEMBL5188939 Ki = 186.0 nM 6.73
CHEMBL5199191 Ki = 250.0 nM 6.60
CHEMBL5199531 Ki = 295.0 nM 6.53
CHEMBL5173920 Ki = 393.0 nM 6.41
CHEMBL5192027 Ki = 605.0 nM 6.22
CHEMBL5186708 Ki = 612.0 nM 6.21
CHEMBL5192248 Ki = 773.0 nM 6.11
CHEMBL5184362 Ki - -
CHEMBL5208709 Ki - -
CHEMBL5177782 Ki - -
CHEMBL5174547 Ki - -
CHEMBL5189565 Ki - -
CHEMBL5184334 Ki - -