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Assay Detail

CHEMBL5136862

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Time dependent inhibition of CYP1A2 in pooled human liver microsomes using midazolam as substrate preincubated for 30 mins in presence of NADPH followed by substrate addition and measured after 10 mins by LC-MS/MS analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1A2 (CHEMBL3356)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a Potent, Selective, and Brain-Penetrant Rho Kinase Inhibitor and its Activity in a Mouse Model of Huntington's Disease.
Ladduwahetty T, Lee MR, Maillard MC, Cachope R, Todd D, Barnes M, Beaumont V, Chauhan A, Gallati C, Haughan AF, Kempf G, Luckhurst CA, Matthews K, McAllister G, Mitchell P, Patel H, Rose M, Saville-Stones E, Steinbacher S, Stott AJ, Thatcher E, Tierney J, Urbonas L, Munoz-Sanjuan I, Dominguez C.
J Med Chem (2022) 65 : 9819 -9845
PubMed 35816678 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.96 5.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5206259 1 5.96
CHEMBL5208605 1 5.96
CHEMBL5176687 1 -
CHEMBL5200987 1 -
CHEMBL5209466 1 -
CHEMBL5188032 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5206259 IC50 = 1100.0 nM 5.96
CHEMBL5208605 IC50 = 1100.0 nM 5.96
CHEMBL5176687 IC50 > 10000.0 nM -
CHEMBL5200987 IC50 > 8800.0 nM -
CHEMBL5209466 IC50 > 10000.0 nM -
CHEMBL5188032 IC50 > 10000.0 nM -