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Assay Detail

CHEMBL5136915

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-epibatidine from human alpha3beta4 nAChR expressed in SH-EP1 cells membrane assessed as inhibition constant preincubated for 5 mins followed by overnight incubation with [3H]-epibatidine by gamma liquid scintilation counting analysis
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-EP1
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha3/beta4 (CHEMBL1907594)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

From 2-Triethylammonium Ethyl Ether of 4-Stilbenol (MG624) to Selective Small-Molecule Antagonists of Human α9α10 Nicotinic Receptor by Modifications at the Ammonium Ethyl Residue.
Bavo F, Pallavicini M, Pucci S, Appiani R, Giraudo A, Eaton B, Lucero L, Gotti C, Moretti M, Whiteaker P, Bolchi C.
J Med Chem (2022) 65 : 10079 -10097
PubMed 35834819 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 6.38 7.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5177606 1 7.48
CHEMBL5176101 1 7.36
CHEMBL5190813 1 7.11
CHEMBL5182114 1 7.02
CHEMBL5197631 1 6.83
CHEMBL5192210 1 6.80
CHEMBL5200914 1 6.80
CHEMBL5181363 1 6.75
CHEMBL5170445 1 6.59
CHEMBL5193899 1 6.54
CHEMBL5171118 1 6.43
CHEMBL1257065 STILONIUM IODIDE 2.0 1 6.36
CHEMBL5184266 1 6.26
CHEMBL5202770 1 6.24
CHEMBL5174508 1 6.18
CHEMBL5182514 1 6.11
CHEMBL5186396 1 6.10
CHEMBL5176544 1 6.06
CHEMBL5193143 1 5.90
CHEMBL5191958 1 5.82
CHEMBL5176690 1 5.80
CHEMBL5178473 1 5.57
CHEMBL5201239 1 5.55
CHEMBL5195695 1 5.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5177606 Ki = 33.0 nM 7.48
CHEMBL5176101 Ki = 44.0 nM 7.36
CHEMBL5190813 Ki = 77.0 nM 7.11
CHEMBL5182114 Ki = 95.0 nM 7.02
CHEMBL5197631 Ki = 147.0 nM 6.83
CHEMBL5192210 Ki = 158.0 nM 6.80
CHEMBL5200914 Ki = 159.0 nM 6.80
CHEMBL5181363 Ki = 176.0 nM 6.75
CHEMBL5170445 Ki = 259.0 nM 6.59
CHEMBL5193899 Ki = 285.0 nM 6.54
CHEMBL5171118 Ki = 368.0 nM 6.43
CHEMBL1257065 STILONIUM IODIDE Ki = 433.0 nM 6.36
CHEMBL5184266 Ki = 546.0 nM 6.26
CHEMBL5202770 Ki = 576.0 nM 6.24
CHEMBL5174508 Ki = 662.0 nM 6.18
CHEMBL5182514 Ki = 783.0 nM 6.11
CHEMBL5186396 Ki = 794.0 nM 6.10
CHEMBL5176544 Ki = 875.0 nM 6.06
CHEMBL5193143 Ki = 1270.0 nM 5.90
CHEMBL5191958 Ki = 1527.0 nM 5.82
CHEMBL5176690 Ki = 1580.0 nM 5.80
CHEMBL5178473 Ki = 2700.0 nM 5.57
CHEMBL5201239 Ki = 2800.0 nM 5.55
CHEMBL5195695 Ki = 3490.0 nM 5.46