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Assay Detail

CHEMBL5137234

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-nisoxetine from NET in Sprague-Dawley rat brain prefrontal cortex incubated for 180 mins by radioligand binding assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Norepinephrine transporter (CHEMBL304)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Illuminating the norepinephrine transporter: fluorescent probes based on nisoxetine and talopram.
Camacho-Hernandez GA, Casiraghi A, Rudin D, Luethi D, Ku TC, Guthrie DA, Straniero V, Valoti E, Schütz GJ, Sitte HH, Newman AH.
RSC Med Chem (2021) 12 : 1174 -1186
PubMed 34355183 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 7.91 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL295467 NISOXETINE 2.0 1 8.82
CHEMBL299233 TALOPRAM 2.0 1 8.35
CHEMBL5188712 1 7.37
CHEMBL196760 1 7.10
CHEMBL5170876 1 -
CHEMBL5176356 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL295467 NISOXETINE Ki = 1.5 nM 8.82
CHEMBL299233 TALOPRAM Ki = 4.5 nM 8.35
CHEMBL5188712 Ki = 43.0 nM 7.37
CHEMBL196760 Ki = 79.0 nM 7.10
CHEMBL5170876 Ki > 10000.0 nM -
CHEMBL5176356 Ki > 10000.0 nM -