Assay Detail
Binding
CHEMBL5139876
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal 3xFlag-His6-tagged SARS-CoV-2 papain-like protease nsp3 (1564 to 1878 residues) expressed in baculovirus infected Sf9 insect cells using Pro3 as substrate by fluorescence based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1ab (CHEMBL4523582) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
Publication
Progress and Challenges in Targeting the SARS-CoV-2 Papain-like Protease.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 6.14 | 6.58 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL599894 | — | — | 1 | 6.58 |
| CHEMBL290904 | — | — | 1 | 6.41 |
| CHEMBL1397270 | — | — | 1 | 6.28 |
| CHEMBL227075 | DIHYDROTANSHINONE I | — | 1 | 6.23 |
| CHEMBL15192 | LAPACHONE | 2.0 | 1 | 6.21 |
| CHEMBL187460 | CRYPTOTANSHINONE | — | 1 | 5.87 |
| CHEMBL187266 | TANSHINONE IIA | — | 1 | 5.80 |
| CHEMBL549695 | — | — | 1 | 5.75 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL599894 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL290904 | — | IC50 | = | 390.0 | nM | 6.41 |
| CHEMBL1397270 | — | IC50 | = | 530.0 | nM | 6.28 |
| CHEMBL227075 | DIHYDROTANSHINONE I | IC50 | = | 590.0 | nM | 6.23 |
| CHEMBL15192 | LAPACHONE | IC50 | = | 610.0 | nM | 6.21 |
| CHEMBL187460 | CRYPTOTANSHINONE | IC50 | = | 1336.0 | nM | 5.87 |
| CHEMBL187266 | TANSHINONE IIA | IC50 | = | 1571.0 | nM | 5.80 |
| CHEMBL549695 | — | IC50 | = | 1789.0 | nM | 5.75 |