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Assay Detail

CHEMBL5140630

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of microRNA in human HeLa cells transfected with pCI-neo-luciferase-miR-21 plasmid assessed as change in luciferase signal incubated for 24 hrs by luciferase reporter assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.
Peng T, He Y, Wang T, Yu J, Ma X, Zhou Z, Sheng Y, Li L, Peng H, Li S, Zou J, Yuan Y, Zhao Y, Shi H, Li F, Liu W, Hu K, Lu X, Lu X, Zhang G, Wang F.
J Med Chem (2022) 65 : 11010 -11033
PubMed 35695407 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 5.43 5.86

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5170759 1 5.86
CHEMBL5178502 1 5.73
CHEMBL5180444 1 5.52
CHEMBL5175086 1 5.22
CHEMBL5179203 1 5.13
CHEMBL5189546 1 5.13

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5170759 EC50 = 1380.0 nM 5.86
CHEMBL5178502 EC50 = 1850.0 nM 5.73
CHEMBL5180444 EC50 = 2990.0 nM 5.52
CHEMBL5175086 EC50 = 5980.0 nM 5.22
CHEMBL5179203 EC50 = 7340.0 nM 5.13
CHEMBL5189546 EC50 = 7440.0 nM 5.13