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Assay Detail

CHEMBL5141282

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human U-937 cells harboring JAK3 M511135 mutant assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type U-937
Confidence 1 — Organism
Curated By Autocuration

Target

U-937 (CHEMBL612794)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery of Hexahydrofuro[3,2-<i>b</i>]furans as New Kinase-Selective and Orally Bioavailable JAK3 Inhibitors for the Treatment of Leukemia Harboring a JAK3 Activating Mutant.
Li S, Si H, Song X, Lei C, He X, Wang J, Liu Y, Zhou Y, Song JG, Peng L, Tang X, Chan S, Ren X, Tu Z, Li Z, Wang Z, Zhang Z, Ding K.
J Med Chem (2022) 65 : 10674 -10690
PubMed 35860875 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.36 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5174210 1 7.82
CHEMBL4085457 RITLECITINIB 4.0 1 7.71
CHEMBL5193702 1 7.70
CHEMBL5176661 1 7.52
CHEMBL5192736 1 7.47
CHEMBL5191734 1 7.45
CHEMBL5201705 1 7.39
CHEMBL5186937 1 7.36
CHEMBL5180117 1 7.30
CHEMBL221959 TOFACITINIB 4.0 1 6.91
CHEMBL5185440 1 6.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5174210 IC50 = 15.2 nM 7.82
CHEMBL4085457 RITLECITINIB IC50 = 19.5 nM 7.71
CHEMBL5193702 IC50 = 20.2 nM 7.70
CHEMBL5176661 IC50 = 30.0 nM 7.52
CHEMBL5192736 IC50 = 33.8 nM 7.47
CHEMBL5191734 IC50 = 35.9 nM 7.45
CHEMBL5201705 IC50 = 40.7 nM 7.39
CHEMBL5186937 IC50 = 44.0 nM 7.36
CHEMBL5180117 IC50 = 50.3 nM 7.30
CHEMBL221959 TOFACITINIB IC50 = 122.4 nM 6.91
CHEMBL5185440 IC50 = 527.4 nM 6.28