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Assay Detail

CHEMBL5142534

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ARC-1063 fluorescent probe binding to recombinant human PKAC-alpha incubated for 1 hr by time-gated luminescence intensity based displacement assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cAMP-dependent protein kinase catalytic subunit alpha (CHEMBL4101)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Construction of Covalent Bisubstrate Inhibitor of Protein Kinase Reacting with Cysteine Residue at Substrate-Binding Site.
Sõrmus T, Lavogina D, Teearu A, Enkvist E, Uri A, Viht K.
J Med Chem (2022) 65 : 10975 -10991
PubMed 35960538 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.58 8.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5188777 1 8.25
CHEMBL5201435 1 7.62
CHEMBL5174274 1 6.40
CHEMBL5189362 1 6.10
CHEMBL5174535 1 5.92
CHEMBL5179913 1 5.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5188777 IC50 = 5.6 nM 8.25
CHEMBL5201435 IC50 = 24.0 nM 7.62
CHEMBL5174274 IC50 = 400.0 nM 6.40
CHEMBL5189362 IC50 = 790.0 nM 6.10
CHEMBL5174535 IC50 = 1200.0 nM 5.92
CHEMBL5179913 IC50 = 6100.0 nM 5.21