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Assay Detail

CHEMBL5142544

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Irreversible inhibition of ARC-1063 fluorescent probe binding to recombinant human PKAC-alpha incubated for 22 hrs followed by probe addition at 20 nM and further incubated for 1 hr by time-gated luminescence intensity based displacement assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cAMP-dependent protein kinase catalytic subunit alpha (CHEMBL4101)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Construction of Covalent Bisubstrate Inhibitor of Protein Kinase Reacting with Cysteine Residue at Substrate-Binding Site.
Sõrmus T, Lavogina D, Teearu A, Enkvist E, Uri A, Viht K.
J Med Chem (2022) 65 : 10975 -10991
PubMed 35960538 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.69 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5174535 1 6.10
CHEMBL5174274 1 5.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5174535 IC50 = 800.0 nM 6.10
CHEMBL5174274 IC50 = 5300.0 nM 5.28