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Assay Detail

CHEMBL5144864

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-inflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as reduction in IL1beta protein expression level after 24 hrs by ELISA
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Antimycobacterial and anti-inflammatory activities of thiourea derivatives focusing on treatment approaches for severe pulmonary tuberculosis.
Calixto SD, Simão TLBV, Palmeira-Mello MV, Viana GM, Assumpção PWMC, Rezende MG, do Espirito Santo CC, de Oliveira Mussi V, Rodrigues CR, Lasunskaia E, de Souza AMT, Cabral LM, Muzitano MF.
Bioorg Med Chem (2022) 53 : 116506 -116506
PubMed 34890996 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.40 4.84

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL521 IBUPROFEN 4.0 1 4.84
CHEMBL5202664 1 4.48
CHEMBL5202128 1 4.32
CHEMBL5197941 1 4.25
CHEMBL5176925 1 4.10
CHEMBL3633777 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL521 IBUPROFEN IC50 = 14400.0 nM 4.84
CHEMBL5202664 IC50 = 33200.0 nM 4.48
CHEMBL5202128 IC50 = 47900.0 nM 4.32
CHEMBL5197941 IC50 = 55700.0 nM 4.25
CHEMBL5176925 IC50 = 80200.0 nM 4.10
CHEMBL3633777 IC50 >= 200000.0 nM -