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Assay Detail

CHEMBL5146067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Tag2-PD1/Tag1-PD-L1 (unknown origin) protein-protein interaction preincubated for 15 mins followed by addition of anti-Tag1-Eu3+ and anti-Tag2-XL665 and measured after 2 hrs by HTRF analysis
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Discovery of 4-phenylindolines containing a (5-cyanopyridin-3-yl)methoxy moiety as potent inhibitors of the PD-1/PD-L1 interaction.
Meng Y, Chu C, Niu X, Cheng L, Wu D, Liu L, Zhang S, Li T, Hou Y, Liu Y, Qin M.
Bioorg Med Chem Lett (2022) 63 : 128647 -128647
PubMed 35231577 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.75 7.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5175245 1 7.27
CHEMBL5184588 1 7.22
CHEMBL5201125 1 7.13
CHEMBL5181918 1 7.06
CHEMBL5189268 1 6.98
CHEMBL5191926 1 6.94
CHEMBL5199860 1 6.91
CHEMBL5181313 1 6.89
CHEMBL5199681 1 6.88
CHEMBL5205437 1 6.81
CHEMBL4749782 1 6.79
CHEMBL5198702 1 6.72
CHEMBL5209536 1 6.64
CHEMBL5189706 1 6.60
CHEMBL5190181 1 6.51
CHEMBL5200124 1 6.42
CHEMBL5181413 1 6.20
CHEMBL5172837 1 6.20
CHEMBL5185877 1 6.06
CHEMBL5198826 1 -
CHEMBL5197289 1 -
CHEMBL5172854 1 -
CHEMBL5191650 1 -
CHEMBL5206183 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5175245 IC50 = 53.2 nM 7.27
CHEMBL5184588 IC50 = 60.1 nM 7.22
CHEMBL5201125 IC50 = 74.6 nM 7.13
CHEMBL5181918 IC50 = 87.3 nM 7.06
CHEMBL5189268 IC50 = 104.5 nM 6.98
CHEMBL5191926 IC50 = 115.7 nM 6.94
CHEMBL5199860 IC50 = 124.1 nM 6.91
CHEMBL5181313 IC50 = 127.8 nM 6.89
CHEMBL5199681 IC50 = 132.1 nM 6.88
CHEMBL5205437 IC50 = 154.2 nM 6.81
CHEMBL4749782 IC50 = 160.6 nM 6.79
CHEMBL5198702 IC50 = 192.1 nM 6.72
CHEMBL5209536 IC50 = 229.2 nM 6.64
CHEMBL5189706 IC50 = 251.5 nM 6.60
CHEMBL5190181 IC50 = 307.7 nM 6.51
CHEMBL5200124 IC50 = 378.4 nM 6.42
CHEMBL5181413 IC50 = 625.7 nM 6.20
CHEMBL5172837 IC50 = 635.2 nM 6.20
CHEMBL5185877 IC50 = 875.7 nM 6.06
CHEMBL5198826 IC50 > 5000.0 nM -
CHEMBL5197289 IC50 > 5000.0 nM -
CHEMBL5172854 IC50 > 5000.0 nM -
CHEMBL5191650 IC50 > 5000.0 nM -
CHEMBL5206183 IC50 > 5000.0 nM -